SRY Rabbit Polyclonal Antibody

CAT#: AP06716PU-N

SRY rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of SRY antibody in extracts from HeLa cells.

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  Immunhistochemistry (IHC) analysis of SRY antibody on paraffin embedded sections

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 50-100 of Human SRY
• Specificity: This antibody detects endogenous levels of SRY protein.
  (region surrounding Leu80)
• Formulation: PBS, pH 7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% by SDS-PAGE)
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 37 kDa
• Gene Name: sex determining region Y
• Database Link:
  Entrez Gene 6736 Human
  UniProt ID Q05066
• Background: SRY (sex-determining region Y protein) is a transcriptional activator required for male sex determination in mammals. This protein, also referred to as testis-determining factor (TDF), is an HMG box protein that initiates the formation of testis from undifferentiated gonad. The DNA-binding activity of SRY is required for normal testis formation. This DNA-binding activity is thought to be regulated by PKA, which phosphorylates SRY in vivo. Mutations in SRY have been associated with 46,XY gonadal dysgenesis, in which the gonads fail to develop in XY phenotypic females.
• Synonyms: TDF; TDY