beta Arrestin 1 (ARRB1) Rabbit Polyclonal Antibody

CAT#: AP06496PU-N

beta Arrestin 1 (ARRB1) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analzysis of Arrestin-β-1 antibody at 1/500 dilution Lane 1: Hela whole cell lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate

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  Immunohistochemistry (IHC) analyzes of Arrestin 1 antibody in paraffin-embedded human brain tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
  Immunofluorescence: 1/50-1/200.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 390-440 of Human Arrestin-β-1.
• Specificity: This antibody detects endogenous levels of Arrestin 1 protein.
  (region surrounding Glu406)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 50 kDa
• Gene Name: arrestin beta 1
• Database Link:
  Entrez Gene 408 Human
  UniProt ID P49407
• Background: Beta Arrestin 1 is a member of a family of proteins that are widely expressed but especially abundant in the central nervous system. Serving as an adaptor or scaffold molecule, beta Arrestin 1 is essential for mitogenic signaling. Beta Arrestin 1 has important roles in the cytoplasm and at the plasma membrane in the desensitization and internalization of G protein coupled receptors (GPCRs) and is increasingly appreciated to play an important role in the endocytosis and signaling of GPCRs. Beta Arrestin 1 in the cytosol is phosphorylated by ERK1 and 2 on serine 412 in a negative feedback mechanism and binds to the phosphorylated receptors at the plasma membrane.
• Synonyms: ARR1, Beta-arrestin-1