Cardiac Troponin I (TNNI3) Rabbit Polyclonal Antibody

CAT#: AP06440PU-N

Cardiac Troponin I (TNNI3) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of TNNI3 antibody in extracts from mouse heart.

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  Immunohistochemistry (IHC) analyzes of TNNI3 antibody in paraffin-embedded human skeletal muscle tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
  Immunofluorescence: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 100-150 of Human Troponin I3.
• Specificity: This antibody detects endogenous levels of TNNI3 protein.
  (region surrounding Gly136)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 28 kDa
• Gene Name: troponin I3, cardiac type
• Database Link:
  Entrez Gene 7137 Human
  UniProt ID P19429
• Background: Troponin I is part of a heteromeric complex playing an important role in the regulation of skeletal and cardiac muscle contraction. It consists of three subunits, troponin I (TnI), troponin T (TnT) and troponin C (TnC). Each subunit is responsible for part of troponin complex function. TnI inhibits ATPase activity of acto myosin and TnT and TnI are present in cardiac muscles in different forms than in skeletal muscles. Only one tissue specific isoform of TnI is described for cardiac muscle tissue (cTnI) and this is expressed only in myocardium.
• Synonyms: TNNI3, TNNC1