cPLA2 beta (PLA2G4B) Rabbit Polyclonal Antibody

CAT#: AP06422PU-N

cPLA2 beta (PLA2G4B) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of c-PLA2 antibody in extracts from HeLa cells treated with TNF-a 20ng/ml 30' or NIH/3T3 cells treated with TNF-a 20ng/ml 30'.

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  Immunohistochemistry analyzes of c-PLA2 antibody in paraffin-embedded human colon carcinoma tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunofluorescence: 1/50-1/200.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 480-530 of Human c-PLA2.
• Specificity: This antibody detects endogenous levels of c-PLA2 protein.
  (region surrounding asn499)
• Formulation: Phosphate buffered saline (PBS), pH 7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction(> 95% pure by SDS-PAGE).
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~100 kDa
• Gene Name: phospholipase A2 group IVB
• Database Link:
  Entrez Gene 100137049 Human
  UniProt ID P0C869
• Background: Phospholipase A2s (PLA2s) constitute a family of esterases that hydrolyze the sn-2-acyl ester bond in glycerophospholipid molecules. These enzymes are generally calcium-dependent and have been found both intra- and extracellularly. By hydrolyzing the sn-2 bond in glycerophospholipids, PLA2s release fatty acids. One such fatty acid, arachidonic acid, generates the substrates for the initiation of the arachidonic acid cascade that produces various eicosanoids (i.e. prostaglandins, leukotrienes and thromboxanes), many of which are potent mediators of inflammation. PLA2s include both the relatively low molecular weight type I and type II enzymes and the form known as cytoplasmic PLA2 (cPLA2). cPLA2 is present in the cytosol of various cells and tissues including platelets, macrophages and monoblasts; and preferentially hydrolyzes the sn-2 position of phospholipid molecules, releasing free arachidonate.
• Synonyms: cPLA2-beta, phospholipase A2 beta