Cytokeratin 10 (KRT10) Rabbit Polyclonal Antibody

CAT#: AP06194PU-N

Cytokeratin 10 (KRT10) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of Cytokeratin 10 antibody at 1/500 dilution: Lane 1: HeLa whole cell lysate Lane 2: Raw264.7 whole cell lysate Lane 3: NIH-3T3 whole cell lysate Lane 4: Rat brain tissue lysate

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunofluorescence: 1/50-1/200.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 130-180 of Human Cytokeratin 10.
• Specificity: This antibody detects endogenous levels of Cytokeratin 10 protein.
  (region surrounding Tyr160)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 59 kDa
• Gene Name: keratin 10
• Database Link:
  Entrez Gene 3858 Human
  UniProt ID P13645
• Background: Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation which is directly applicable to the characterization of malignant tumors. Cytokeratins 10 and 13 are present in the cytoskeletal region of a subset of squamous cell carcinomas. Cytokeratin 10 is a heterotetramer of two type I and two type II keratins, is generally associated with keratin 1, and is seen in all suprabasal cell layers including stratum corneum.
• Synonyms: Cytokeratin-10, CK10, CK-10, Keratin-10, KRT10, KPP, K10