esxA Rabbit Polyclonal Antibody

CAT#: AP03011PU-N

esxA rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 1. Western Blotting analysis (reducing conditions) of recombinant protein EsaT-6 using polyclonal anti-EsaT-6 antibody (AP03011PU-N): Lane 1, 3, 5:</em> cell lysates of non-transfected E. coli (Negative Controls). Lane 2, 4, 6:</em> Cell

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western Blotting (Reducing conditions): 0.2-1 μg/ml.
• Reactivities: Mycobacterium tuberculosis
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: EsaT-6 (Rv3875) of Mycobacterium tuberculosis.
• Specificity: This polyclonal antibody reacts with EsaT-6, a 11,8 kDa antigen encoded by gene Rv3875 of Mycobacterium tuberculosis.
  The antibody recognizes EsaT-6 of Mycobacterium tuberculosis H37Rv and Mycobacterium bovis. It reacts also with recombinant antigen produced in Escherichia coli.
• Formulation: PBS, pH~7.4
  State: Aff - Purified
  State: Liquid purified IgG fraction (> 95% pure by SDS-PAGE)
  Preservative: 15 mM Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID P0A564
• Background: The ESAT6 antigen from Mycobacterium tuberculosis is a dominant target for cell mediated immunity in the early phase of tuberculosis (TB) in TB patients as well as in various animal models. ESAT6 is not found in M. bovis BCG. Its function is not known but it elicits high level of IFN-gamma from memory effector cells during the first phase of a protective immune response.
• Synonyms: esxA, esaT-6, Rv3875, MT3989, MTV027.10