Muscarinic Acetylcholine Receptor M5 (CHRM5) Rabbit Polyclonal Antibody

CAT#: AP01305PU-N

Muscarinic Acetylcholine Receptor M5 (CHRM5) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of Adenosine mAChR M5 antibody in extracts from Jurkat cells.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
  Immunofluorescence: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 291-340 of Human mAChR M5.
• Specificity: This antibody detects endogenous levels of mAChR M5 protein. (region surrounding Pro224)
• Formulation: Phosphate buffered saline (PBS), pH 7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05 Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 60 kDa
• Gene Name: cholinergic receptor muscarinic 5
• Database Link:
  Entrez Gene 1133 Human
  UniProt ID P08912
• Background: The muscarinic acetylcholine receptors (mAChR) mediate a variety of cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels. The mAChRs transducer signals by coupling to G-proteins, which then modulate several downstream effector proteins and ion channels. Five mAChR subtypes have been identified, designated M1 to M5. The five receptor subtypes show distinct patterns of tissue distribution, as well as distinct pharmacological and functional properties. The amino acid sequence of each mAChR subtype reflects a structure that is characteristic of G-protein coupled receptors, consisting of seven highly conserved transmembrane segments and a large intracellular region unique to each subtype, which constitutes the effector-coupling domain.
• Synonyms: CHRM5, mAChR M5, mAChR-M5