CD153 (TNFSF8) Rabbit Polyclonal Antibody

CAT#: AP01279PU-N

CD153 (TNFSF8) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of CD30-L antibody at 1/500 dilution Lane 1: MCF-7 whole cell lysate Lane 2: Mouse liver tissue lysate Lane 3: Rat liver tissue lysate

Specifications

Product Data

• Applications: ELISA, IF, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunofluorescence: 1/50 - 1/200.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 80-130 of Human CD30-L.
• Specificity: This antibody detects endogenous levels of CD30-L protein.
  (region surrounding Gln103)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction (>95% pure by SDS-PAGE)
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-Chromatography using epitope-specific immunogen
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 38 kDa
• Database Link:
  Entrez Gene 944 Human
  UniProt ID P32971
• Background: The ligand for CD30 is CD30L (CD153). The binding of CD30 to CD30L mediates pleiotropic effects including cell proliferation, activation, differentiation, and apoptotic cell death. CD30 has a critical role in the pathophysiology of Hodgkin's disease and other CD30+ lymphomas. CD30 acts as a costimulatory molecule in thymic negative selection. In addition to its expression on Hodgkin's and Reed-Sternberg cells, CD30 is also found in some non-Hodgkin's lymphomas (including Burkitt's lymphomas), virus-infected T and B cells, and on normal T and B cells after activation. In T cells, CD30 expression is present on a subset of T cells that produce Th2-type cytokines and on CD4+/CD8+ thymocytes that coexpress CD45RO and the IL4 receptor.
• Synonyms: TNFSF8, CD30 Ligand, CD30LG, CD30-L