Histone H2A.X (H2AFX) Rabbit Polyclonal Antibody

CAT#: AP01278PU-N

Histone H2A.X (H2AFX) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis with extracts from human HT-29 cells using Histone H2A.X antibody.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunofluorescence: 1/50-1/200
  Immunohistochemistry on Paraffin sections: 1/50-1/200.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 100-150 of Human Histone H2A.X.
• Specificity: This antibody detects endogenous levels of Histone H2A.X protein (region surrounding Lys128).
• Formulation: Phosphate buffered saline (PBS), pH 7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 15 kDa
• Gene Name: H2A histone family member X
• Database Link:
  Entrez Gene 3014 Human
  UniProt ID P16104
• Background: H2A.X is a member of the histone H2A family, 1 of 5 families of histone proteins involved in nucleosomal organization of chromatin. H2A.X is synthesised in G1 as well as S phase and is known to be important for recombination between immunoglobulin switch regions. A very early step in the response of mammalian cells to DNA double-strand breaks is the phosphorylation of histone H2A.X at serine 139 at the sites of DNA damage. There is a predicted acetylation at residue 1 and ubiquitination at reside 119. Phosphorylated H2A.X promotes DNA repair and maintains genomic stability.
• Synonyms: H2a/x, H2AFX, H2AX

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Systemic lupus erythematosus