Endothelin A Receptor (EDNRA) Rabbit Polyclonal Antibody

CAT#: AP01200PU-N

Endothelin A Receptor (EDNRA) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of ETAR antibody in extracts from HepG2 cells.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: Western Blot: 1/1000.
  Immunofluorescence: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 402-452 of Human ETAR.
• Specificity: This antibody detects endogenous levels of Endothelin Receptor A (ETAR) protein. (region surrounding asn427)
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Predicted Protein Size: ~50 kDa
• Gene Name: endothelin receptor type A
• Database Link:
  Entrez Gene 1909 Human
  UniProt ID P25101
• Background: Endothelin receptor A (ETAR), also known as EDNRA, ET1 receptor, ETA, EDN1 and ET-AR, is a member of the guanine-binding regulatory protein-coupled receptor family. ETAR binds endothelins and has the highest affinity for its ligand, ET1, as compared to the ETBR receptor. Both ET receptors, ETAR and ETBR, are activated by ET1, which results in inhibition of active lens sodiumpotassium transport. Activation of the ET receptors also causes an increase in cytoplasmic calcium concentration in cultured lens epithelial cells. In addition, ETAR induces arachidonic acid accumulation. ETAR has seven hydrophobic transmembrane domains and is expressed in aorta, lung, atrium, kidney, placenta and prostate. Specifically, placental vascular smooth muscle cells (PVSMCs) exclusively express ETAR.
• Synonyms: Endothelin-1 receptor, hET-AR, ETA-R, ET-A, EDNRA, ETA, ETRA