Pcdhga1 (808-931, Cytopl. Dom.) Mouse Monoclonal Antibody [Clone ID: S159-5]

CAT#: AM60060PU-N

Pcdhga1 (808-931, Cytopl. Dom.) mouse monoclonal antibody, clone S159-5, Purified

Product Images

Western blot of rat brain membrane lysates showing the detection of ~100 kDa. Protocadherin Gamma (pan) protein using Anti-Protocadherin Gamma (pan) Antibody [S159-5] (Cat.-No AM60060PU). Lane 1: MW ladder. Lane 2: Anti- Protocadherin Gamma (pan) (1:1000). Load: 10 µg RBM per lane. Block: 5% milk + TBST O/N at 4°C. Primary antibody: Mouse Anti- Protocadherin Gamma (pan) monoclonal antibody [S159-5] (Cat.-No AM60060PU) incubated for 60 min at RT. Secondary antibody: Goat Anti-Mouse HRP antibody at 1:200 for 60min at RT. Development: ECL solution for 6min at RT. Predicted/Observed size: ~ 100 kDa. Other band(s):~None.

Specifications

Product Data

• Clone Name: S159-5
• Applications: IF, WB
• Recommended Dilution: Western blot: 1/1000, 1 μg/ml was sufficient for detection of PCDHG (pan) in 20 μg of Rat brain lysate by colorimetric immunoblot analysis using HRP conjugated secondary antibody.
  Immunocytochemistry.
  
• Reactivities: Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Fusion protein corresponding to amino acids 808-931 (C-terminal cytoplasmic constant domain) of mouse Protocadherin-gamma-A1 that is shared by all 22 Gamma-protocadherins (A-subfamily: amino acids ~807-930, B-subfamily: amino acids ~789-912 and C subfamily).
• Specificity: This antibody detects a ~100 kDa protein. Cross-reacts with all Gamma- protocadherin-A, -B and -C proteins.
• Formulation: PBS pH 7.4, 50% Glycerol, 0.09% Sodium Azide
  State: Purified
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Protein G Chromatography
• Conjugation: Unconjugated
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Database Link:
  Entrez Gene 93709 Mouse
  UniProt ID Q91XZ0
• Background: The protocadherin gamma gene cluster is one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes.
  The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes.
• Synonyms: Protocadherin gamma, PCDH gamma, Gamma protocadherin