GARS Mouse Monoclonal Antibody [Clone ID: AT4E10]

CAT#: AM50342PU-S

GARS mouse monoclonal antibody, clone AT4E10, Purified

Product Images

The cell lysates of Hela and 293T (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GARS antibody 1:500. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Product Data

• Clone Name: AT4E10
• Applications: ELISA, WB
• Recommended Dilution: The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant human GARS (43-289aa) purified from E. coli.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein-A affinity chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: glycyl-tRNA synthetase
• Database Link:
  Entrez Gene 2617 Human
  UniProt ID P41250
• Background: GARS, also known as glycyl-tRNA synthetase, is one of the aminoacyl-tRNA synthetase that charge tRNAs with their cognate amino acids. Defects in the gene encoding GlyRS is the cause of Charcot-Marie-Tooth disease type 2D (CMT2D), which is an autosomal dominant inherited disease characterized by severe weakness, atrophy and absence of deep tendon reflexes in the upper extremities. Defects in the GlyRS gene is also the cause of distal hereditary muscular neuropathy type V (HMN5), a disease similar to CMT2D, though the distal sensory involvement is less severe in HMN5 patients.
• Synonyms: GARS, Glycine-tRNA ligase, GlyRS, AP-4-A synthetase, SMAD1

Reference Data

• Protein Pathways: Aminoacyl-tRNA biosynthesis