hCG beta (CGB3) Mouse Monoclonal Antibody [Clone ID: HCGb/54+ HCGb/459]

CAT#: AM50280PU-T

hCG beta (CGB3) mouse monoclonal antibody, clone HCGb/54+ HCGb/459, Purified

Product Images

Formalin-Fixed, Paraffin-Embedded Human Placenta stained with hCG beta Antibody (Clone HCGb/54+HCGb/459). Note specific membrane staining.

Specifications

Product Data

• Clone Name: HCGb/54+ HCGb/459
• Applications: IHC, WB
• Recommended Dilution: Western Blot: 0.5-1 µg/ml.
  Immunohistochemsitry on Frozen and formalin-Fixed Paraffin Sections: 0.5-1 µg/ml for 30 min RT.
  Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
  Positive Control: Placenta.
• Reactivities: Human
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: Recombinant hCG beta protein (HCGb/54 and HCGb/459).
• Specificity: This MAb reacts with a protein of 22kDa, identified as beta sub-unit of HCG. It does not cross react with the alpha sub-unit. HCG is a glycoprotein, which is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the α and β subunits. The α subunit is identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH).
  hCG MAb detects cells and tumors of trophoblastic origin such as choriocarcinoma. Large cell carcinoma and adenocarcinoma of the lung demonstrate anti-hCG positivity in 90% and 60% of cases respectively. 20% of lung squamous cell carcinomas are positive. hCG expression by non-trophoblastic tumors may indicate aggressive behavior.
  Cellular Localization: Cytoplasmic, secreted.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 22 kDa
• Gene Name: chorionic gonadotropin beta subunit 3
• Database Link:
  Entrez Gene 1082 Human
  UniProt ID P0DN86
• Synonyms: Choriogonadotropin subunit beta, CGB, CGB3, beta hCG, hCG-beta