TTF1 (NKX2-1) Mouse Monoclonal Antibody [Clone ID: SPM150]

CAT#: AM50175PU-T

TTF1 (NKX2-1) mouse monoclonal antibody, clone SPM150, Purified

Product Images

Formalin-Paraffin lung adenocarcinoma (10X) stained with TTF-1 Antibody (Clone SPM150).

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  Formalin-Paraffin lung adenocarcinoma (20X) stained with TTF-1 Antibody (Clone SPM150).

Specifications

Product Data

• Clone Name: SPM150
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA: Use BSA free Antibody for coating.
  Flow Cytometry: 0.5-1 µg/million cells.
  Immunofluorescence: 0.5-1 µg/ml.
  Western Blotting: 0.5-1 µg/ml.
  Immunoprecipitation: 1-2 µg/500 µg protein lysate.
  Immunohistochemistry on Frozen and Formalin-Fixed Sections: 0.5-1 µg/ml for 30 minutes at RT.
  Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
  Positive Control: MAD109, MLE-15, H441-4, or H345 cells. Normal thyroid or lung.
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Rat full length TTF-1 recombinant protein.
• Specificity: Recognizes a protein of 40kDa, identified as Thyroid transcription factor-1 (TTF-1).
  TTF-1 is a member of the NKx2 family of homeodomain transcription factors. It is expressed in epithelial cells of the thyroid gland and the lung. Nuclei from liver, stomach, pancreas, small intestine, colon, kidney, breast, skin, testes, pituitary, prostate, and adrenal glands are unreactive. Anti-TTF-1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors, and malignant mesothelioma. It can also be used to differentiate small cell lung carcinoma from lymphoid infiltrates. Loss of TTF-1 expression in non-small cell lung carcinoma has been associated with aggressive behavior of such neoplasms. TTF-1 reactivity is also seen in thyroid malignancies.
  Cellular Localization: Nuclear.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 40 kDa
• Gene Name: NK2 homeobox 1
• Database Link:
  Entrez Gene 7080 Human
  UniProt ID P43699
• Synonyms: NKX2-1, Nkx-2.1, TITF1, Thyroid nuclear factor 1, Thyroid transcription factor 1, TTF-1