Cytokeratin 10 (KRT10) Mouse Monoclonal Antibody [Clone ID: SPM261]

CAT#: AM50129PU-S

Cytokeratin 10 (KRT10) mouse monoclonal antibody, clone SPM261, Purified

Specifications

Product Data

• Clone Name: SPM261
• Applications: IF, IHC, IP, WB
• Recommended Dilution: ELISA: Use BSA free Antibody for coating.
  Flow Cytometry: 0.5-1 µg/million cells.
  Immunofluorescence: 0.5-1 µg/ml.
  Western Blotting: 0.25-0.5 µg/ml.
  Immunoprecipitation: 0.5-1 µg/500 µg protein lysate.
  Immunohistochemistry on Frozen and Formalin-fixed Sections: 0.5-1.0 µg/ml for 30 minutes at RT.
  Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8-9, for 10-20 min followed by cooling at RT for 20 minutes.
  Positive Control: A431, HeLa, MCF7 cells or Esophagus.
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Skin extract of a Human Psoriasis patient.
• Specificity: This MAb recognizes a protein of 56.5kDa, identified as Cytokeratin 10 (CK10).
  Cellular Localization: Cytoplasmic.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 56.5 kDa
• Gene Name: keratin 10
• Database Link:
  Entrez Gene 3858 Human
  UniProt ID P13645
• Background: CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.
• Synonyms: Cytokeratin-10, CK10, CK-10, Keratin-10, KRT10, KPP, K10