PRPS1 Mouse Monoclonal Antibody [Clone ID: AT1E11]

CAT#: AM50064PU-N

PRPS1 mouse monoclonal antibody, clone AT1E11, Purified

Product Images

The lysate of 293T (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PRPS1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Product Data

• Clone Name: AT1E11
• Applications: ELISA, IF, WB
• Recommended Dilution: The antibody has been tested by ELISA, Western blot analysis and Immunofluorescence / Immnunocytochemistry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Recombinant human PRPS1 (1-318aa) purified from E. coli
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein-A affinity chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: phosphoribosyl pyrophosphate synthetase 1
• Database Link:
  Entrez Gene 5631 Human
  UniProt ID P60891
• Background: PRPS1 is an enzyme that catalyzes the phosphoribosylation of ribose 5-phosphate to 5-phosphoribosyl-1-pyrophosphate, which is necessary for purine metabolism and nucleotide biosynthesis. A mutation in PRPS1 may result in PRPS superactivity, a disease characterized by gout and the overproduction of purine nucleotides, uric acid and PRPP. PRPS1 mutations can also lead to a reduction in PRPS1 activity resulting in ARTS syndrome or CMTX5 (Charcot-Marie-Tooth disease X-linked recessive type 5).
• Synonyms: PRS-I, PPRibP

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Pentose phosphate pathway, Purine metabolism