LSM2 Mouse Monoclonal Antibody [Clone ID: 1G7]

CAT#: AM50011PU-N

LSM2 mouse monoclonal antibody, clone 1G7, Purified

Product Images

The cell lysates (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human LSm2 antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Product Data

• Clone Name: 1G7
• Applications: ELISA, WB
• Recommended Dilution: The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000~1:3,000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Recombinant human LSm2 (1-95aa) purified from E. coli
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein-G affinity chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: LSM2 homolog, U6 small nuclear RNA and mRNA degradation associated
• Database Link:
  Entrez Gene 57819 Human
  UniProt ID Q9Y333
• Background: LSM2 is a member of the LSm family of RNA-binding proteins. Component of LSm protein complexes, which are involved in RNA processing and may function in a chaperone-like manner. Component of the cytoplasmic complex which is thought to be involved in mRNA degradation by activating the decapping step. Component of the nuclear LSM2 complex, which is involved in splicing of nuclear mRNAs. LSM2 associates with multiple snRNP complexes containing the U6 snRNA It binds directly to the U6 snRNA and plays a role in the biogenesis and stability of the U6 snRNP and U4/U6 snRNP complexes. It probably also is involved degradation of nuclear pre-mRNA by targeting them for decapping. LSM2 binds specifically to the 3'-terminal U-tract of U6 snRNA. LSM2-LSM8 probably is involved in processing of pre-tRNAs, pre-rRNAs and U3 snoRNA.
• Synonyms: C6orf28, G7B