ACOX1 Mouse Monoclonal Antibody [Clone ID: 153CT43.1.1]

CAT#: AM33416PU-N

ACOX1 mouse monoclonal antibody, clone 153CT43.1.1, Purified

Product Images

Western blot analysis in K562 cell line lysates using ACOX1 Monoclonal Antibody (Clone 153CT43.1.1) (15ug/lane). This demonstrates the ACOX1 antibody detected the ACOX1 protein (arrow). (1 ug/ml)1:390

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  Immunohistochemistry analysis in Formalin Fixed and Paraffin Embedded human colon carcinomastained with ACOX1 Monoclonal Antibody (Clone 153CT43.1.1) followed by peroxidase conjugation of the secondary antibody and DAB staining.

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  Confocal immunofluorescent analysis of ACOX1 Monoclonal Antibody (Clone 153CT43.1.1) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).

Specifications

Product Data

• Clone Name: 153CT43.1.1
• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/100-1/500.
  Immunofluorescence: 1/10-1/50.
  Immunohistochemistry on Paraffin Sections: 1/10-1/50.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: ACOX1 recombinant protein.
• Specificity: This antibody recognizes Human ACOX1.
  Other species not tested.
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.09% (W/V) Sodium Azide
• Concentration: lot specific
• Purification: Protein G Chromatography, followed by dialysis against PBS
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 51 Human
  UniProt ID Q15067
• Background: ACOX1 is the first enzyme of the fatty acid beta-oxidation pathway, which catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. It donates electrons directly to molecular oxygen, thereby producing hydrogen peroxide. Defects in this gene result in pseudoneonatal adrenoleukodystrophy, a disease that is characterized by accumulation of very long chain fatty acids.
  Function: Catalyzes the desaturation of acyl-CoAs to 2-trans- enoyl-CoAs. Isoform 1 shows highest activity against medium-chain fatty acyl-CoAs and activity decreases with increasing chain length. Isoform 2 is active against a much broader range of substrates and shows activity towards very long-chain acyl-CoAs. Isoform 2 is twice as active as isoform 1 against 16-hydroxy- palmitoyl-CoA and is 25% more active against 1,16-hexadecanodioyl- CoA.
  Cellular Location: Peroxisome.
  Tissue Location: Widely expressed with highest levels of isoform 1 and isoform 2 detected in testis. Isoform 1 is expressed at higher levels than isoform 2 in liver and kidney while isoform 2 levels are higher in brain, lung, muscle, white adipose tissue and testis. Levels are almost equal in heart.
• Synonyms: AOX, Palmitoyl-CoA oxidase, Straight-chain acyl-CoA oxidase