p53 (TP53) Mouse Monoclonal Antibody [Clone ID: Pab122]

CAT#: AM33349PU-T

p53 (TP53) mouse monoclonal antibody, clone Pab122, Purified

Specifications

Product Data

• Clone Name: Pab122
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA: Use BSA free Antibody for coating.
  Flow Cytometry: 0.5-1 µg/million cells.
  Immunofluorescence: 0.5-1 µg/ml.
  Western Blotting: 0.5-1.0 µg/ml.
  Immunoprecipitation: 0.5-1 µg/500 µg protein lysate.
  Immunohistochemistry on Frozen Sections: 0.5-1.0 µg/ml for 30 minutes at RT.
  Positive Control: MDA-MB-231 Cells. Breast or Colon carcinoma.
• Reactivities: Canine, Hamster, Human, Monkey, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Recombinant Human wild type p53 protein expressed in E. coli.
• Specificity: Recognizes a conserved determinant of the p53 protein. Its epitope maps near the C-terminus end (aa 370-378) of p53. It has been used successfully to immunoprecipitate p53 oncoprotein complexes with simian virus 40 (SV40) large T antigen. Reportedly, this antibody precipitated the most ATPase-active form of T.p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Mutations involving p53 are found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.
  Cellular Localization: Predominantly nuclear.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 53 kDa
• Gene Name: tumor protein p53
• Database Link:
  Entrez Gene 22059 MouseEntrez Gene 24842 RatEntrez Gene 716170 MonkeyEntrez Gene 7157 Human
  UniProt ID P04637
• Background: p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to mdm2, SV40 T antigen and human papilloma virus E6 protein p53 senses DNA damage and possibly facilitating repair. Mutation involving p53 is found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.
• Synonyms: Cellular tumor antigen p53, Tumor suppressor p53, Phosphoprotein p53, NY-CO-13

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), Apoptosis, Basal cell carcinoma, Bladder cancer, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, Glioma, Huntington's disease, MAPK signaling pathway, Melanoma, Neurotrophin signaling pathway, Non-small cell lung cancer, p53 signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, Small cell lung cancer, Thyroid cancer, Wnt signaling pathway