CD63 (Late Endosomes Marker) Mouse Monoclonal Antibody [Clone ID: SPM524]

CAT#: AM33263PU-S

CD63 (Late Endosomes Marker) mouse monoclonal antibody, clone SPM524, Purified

Product Images

Formalin-paraffin human melanoma stained with CD63 MAb (Clone SPM524)

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  Formalin-paraffin mouse spleen stained with CD63 MAb (Clone SPM524)

Specifications

Product Data

• Clone Name: SPM524
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA: Use BSA Free antibody for coating.
  Flow Cytometry: 0.5-1 µg/10⁶ cells.
  Immunofluorescence: 1-2 µg/ml.
  Western Blotting: 0.5-1 µg/ml.
  Immunoprecipitation: 1-2 µg/500 µg protein lysate.
  Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT.
  Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
  Recommended Positive Control: SK-MEL-28, HL60, THP-1 or NIH/3T3 cells. Melanoma or lymphoma.
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Smooth plasma membrane fraction of MeWo cells.
• Specificity: This Monoclonal antibody recognizes Human and Mouse CD63. Other species not tested.
  Cellular Localization: Cytoplamsic.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 26 kDa (core protein); 30-60 kDa (glycosylated)
• Gene Name: CD63 molecule
• Database Link:
  Entrez Gene 967 Human
  UniProt ID P08962
• Background: The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.
• Synonyms: OMA81H, Granulophysin, Tetraspanin-30, MLA1, TSPAN30, ME491