GFAP Mouse Monoclonal Antibody [Clone ID: SPM507]

CAT#: AM33257PU-S

GFAP mouse monoclonal antibody, clone SPM507, Purified

Product Images

Western blot analysis of GFAP in Human brain lysate using GFAP Antibody (Clone SPM507).

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  Formalin-Paraffin Human brain stained with GFAP Antibody (Clone SPM507). Note cytoplasmic staining.

Specifications

Product Data

• Clone Name: SPM507
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: Flow Cytometry: 0.5 µg/10⁶ cells in 0.1 ml.
  Immunofluorescence: 1-2 µg/ml.
  Western Blot: 0.5-1 µg/ml.
  Immunoprecipitation: 1-2 μg/500 µg protein lysate.
  Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT.
  Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
  Positive Control: Brain or Astrocytoma.
• Reactivities: Bovine, Chicken, Human, Mouse, Porcine, Rabbit
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: GFAP isolated from pig spinal cord.
• Specificity: This Monoclonal antibody recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
  Cellular Localization: Cytoplasmic.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~50 kDa
• Gene Name: glial fibrillary acidic protein
• Database Link:
  Entrez Gene 2670 Human
  UniProt ID P14136
• Background: Glial Fibrillary Acidic Protein (GFAP) is specific to astrocytes (i.e., glial cells) and ependymal cells of the central nervous system.
• Synonyms: Glial Fibrillary Acidic Protein