Collagen IV (COL4A1) Mouse Monoclonal Antibody [Clone ID: 1043]

CAT#: AM33006PU-N

Collagen IV (COL4A1) mouse monoclonal antibody, clone 1043, Purified

Product Images

Immunohistochemistry on frozen section of human colon

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  Immunohistochemistry on paraffin section of human skin

Specifications

Product Data

• Clone Name: 1043
• Applications: FC, IF, IHC, WB
• Recommended Dilution: Western blot: 1/100-1/1000.
  Immunocytochemistry on fixed cells (methanol fixation).
  Flow cytometry.
  Immunohistochemistry on Frozen Sections using a PBS buffer containing 0.1 mM CaCl₂ and 0.1 mM MgCl₂.
  Immunohistochemistry on Paraffin Sections: Pretreatement with 1 mg/ml pepsin in 0.01N HCl for 30 minutes at room temperature is recommended.
  Recommended Dilutions: 1/50–1/100 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified Human type IV Collagen, isolated from Human placenta.
  Antibody derived by fusion of Sp 2/0 mouse myeloma cells with spleen cells from a BALB/c mouse.
• Specificity: This antibody clone 1043 recognizes type IV collagen in the extracellular – basement membranes of normal and pathological tissues.
  
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freeze-thaw cycles.
• Stability: Shelf life: One year from despatch.
• Gene Name: collagen type IV alpha 1 chain
• Database Link:
  Entrez Gene 1282 Human
  UniProt ID P02462
• Background: Type IV collagen is an integral part of most, if not all basement membranes. The extent of expression of the basement membrane component type IV collagen may be correlated with the degree of differentiation of basement membrane producing neoplasms. Neoplastic cells of invasive carcinomas often lack a continuous basement membrane.
  In diagnostic histopathology antibodies to type IV collagen may help to distinguish between non-invasive (benign or in situ) and invasive lesions. Furthermore the antiserum can be of help in the differential diagnosis of bullous lesions in dermatopathology.
• Synonyms: COL4A1