GFAP Mouse Monoclonal Antibody [Clone ID: GA5]

CAT#: AM32829PU-S

GFAP mouse monoclonal antibody, clone GA5, Purified

Product Images

Western blot analysis of GFAP in Human brain lysate using GFAP Antibody (Clone GA5).

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  Formalin-Paraffin Human brain stained with GFAP Antibody (Clone GA-5). Note cytoplasmic staining.

Specifications

Product Data

• Clone Name: GA5
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: ELISA: Use Antibody without BSA for Coating.
  Western Blot: 0.5-1 µg/ml.
  Flow Cytometry: 0.5-1 µg/10⁶ cells.
  Immunofluorescence: 1-2 µg/ml.
  Immunoprecipitation: 1-2 µg/500 µg protein lysate.
  Immunohistochemistry on Cryo Sections: 0.5-1 µg/ml (See also Tobin et. al. for details).
  Immunohistochemistry on Paraffin Sections: 0.5-1 µg/ml for 30 minutes at RT.
  Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
  Recommended Positive Control: Brain or Astrocytoma.
• Reactivities: Bovine, Chicken, Human, Mouse, Porcine, Rabbit, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Glial Fibrillary Protein from Porcine spinal cord
• Specificity: This Monoclonal antibody recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins.
  It labels some astrocytes and some CNS ependymal cells but not oligodendrocytes or neurons.
  Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
  Cellular Localization: Cytoplasmic.
• Formulation: 10mM PBS
  State: Purified
  State: Liquid purified IgG fraction from Bioreactor Concentrate
  Stabilizer: 0.05% BSA
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A/G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~50 kDa
• Gene Name: glial fibrillary acidic protein
• Database Link:
  Entrez Gene 2670 Human
  UniProt ID P14136
• Background: Glial fibrillary acidic protein (GFAP) is a class-III intermediate-filament (IF) protein that is highly specific for cells of astroglial lineage, although its tissue-specific role is speculative. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. The gene is localized to chromosome 17q21. Alternate splicing of this gene generates several transcript variants encoding three different isoforms.
• Synonyms: Glial Fibrillary Acidic Protein