Influenza A H1N1 Mouse Monoclonal Antibody [Clone ID: 11F8]

CAT#: AM32421PU-N

Influenza A H1N1 mouse monoclonal antibody, clone 11F8, Purified

Specifications

Product Data

• Clone Name: 11F8
• Applications: ELISA, IF, WB
• Recommended Dilution: ELISA.
  Western Blot: 1/500-1/5000.
  Immunofluorescence: 1/200-1/1000.
• Reactivities: Influenza A Virus
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Inactivated H1N1 virion
• Specificity: Reacts with H1N1 Influenza A virus.
• Formulation: 0.1M Tris, 0.1M Glycine, 2% Sucrose
  State: Purified
  State: Lyophilized purified powder
  Preservative: None
• Reconstitution Method: Restore with distilled water
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Background: Influenza type A viruses are divided into subtypes based on the antigenic differences of two viral surface proteins, hemagglutinin (H) and neuraminidase (N). On infection of the respiratory tract, the hemagglutinin molecule binds to sialic acid-containing receptors on the epithelial cells resulting in endocytosis. Once the virus has been engulfed, the hemagglutinin allows the viral membrane to fuse with the endosomal membrane. Neuraminidase functions to aid viral release from host cells by cleaving terminal sialic acid residues from carbohydrate moieties on the cell surface.
  Subtype antigenic variations result from a process known as antigenic drift whereby these surface proteins constantly mutate in order to evade the host immune repspone. Subtype A (H1N1) was the cause of Spanish flu that killed approximately 500,000 people between 1918-1919.
• Synonyms: Seasonal Flu H1N1