Cd27 Hamster Monoclonal Antibody [Clone ID: LG.3A10]

CAT#: AM31861PU-N

Cd27 hamster monoclonal antibody, clone LG.3A10, Purified

Product Images

Flow Cytometry: Balb/c Thymocytes labelled with AM31861RP-N including isotypic control. Percentage of cells stained above control: 90%

Specifications

Product Data

• Clone Name: LG.3A10
• Applications: FC, FN, IHC
• Recommended Dilution: ELISA: In combination with LG.8A6, it is possible to develop a double determinant ELISA to detect soluble Mouse CD27.
  Flow Cytometry:
  Functional Studies (stimulating).
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin Sections: This clone (LG.3A10) has been reported to work on Paraffin tissue sections.
• Reactivities: Mouse
• Host: Hamster
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: MCD27 tag expressing clones AR0524 and AR0530 from Armenian Hamster spleen.
  Fusion Partner: SP2/0 Murine Myeloma Partner.
• Specificity: Recognizes Mouse CD27 receptor.
• Formulation: PBS containing 0.02% Sodium Azide as preservative
  State: Purified
  State: Liquid purified IgG fraction from Ascites
• Concentration: lot specific
• Purification: Protein G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Mus musculus CD27 antigen (Cd27), transcript variant 1
• Database Link:
  Entrez Gene 21940 Mouse
  UniProt ID P41272
• Background: CD27 is part of the Tumor Necrosis Factor (TNF) family of receptors and is lymphocyte specific. Murine CD27 is expressed on the great majority of both and T lymphocytes, on a small population of peripheral B cells and on a very small subset of B220+ cells in the bone marrow. Murine CD27 is found on all thymocytes except a subset of CD4-CD8- precursors.
  There are two known basic functions of CD27 signaling. First, the binding of CD27 to CD70, a protein which is also expressed in T and B cells, provides costimulatory signals in lymphocyte proliferation and immunoglobulin production. Second, the association of CD27 with intracellular Siva, a proapoptotic protein, results in the induction of apoptotic events.
• Synonyms: TNFRSF7, CD27L receptor, T14