ZNFN1A2 (IKZF2) (51-107) Hamster Monoclonal Antibody [Clone ID: 22F6]

CAT#: AM26777RP-N

ZNFN1A2 (IKZF2) (51-107) hamster monoclonal antibody, clone 22F6, PE

Product Images

Human peripheral blood lymphocytes were stained with anti-CD4 Pacific BlueTM, fixed and permeabilized with Treg Detection Kit (Miltenyi cat. 130-094-163), and then intracellulary stained with anti-Helios PE (clone 22F6).

Specifications

Product Data

• Clone Name: 22F6
• Applications: FC
• Recommended Dilution: Flow cytometry analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension.
  The content of a vial (1 ml) is sufficient for 100 tests.
• Reactivities: Human, Mouse
• Host: Hamster
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: Peptide coresponding to the amino acids 51-107 of Helios
• Specificity: This antibody recognizes Helios, a transcription factor expressed in some hematopoietic stem cells, and at high levels in thymic-derived regulatory T cells.
• Formulation: Phosphate buffered saline (PBS)
  Label: PE
  State: Liquid purified Ig fraction
  Stabilizer: 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA)
  Preservative: 15 mM sodium azide
  Label: Conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
• Conjugation: PE
• Storage: Store the antibody undiluted at 2-8°C.
  DO NOT FREEZE!
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Gene Name: IKAROS family zinc finger 2
• Database Link:
  Entrez Gene 22807 Human
  UniProt ID Q9UKS7
• Background: Helios, also known as IKZF2 (Ikaros family zinc finger protein 2) is a hematopoietic-specific transcription factor involved in the regulation of lymphocyte development, together with other members of this family, such as Aiolos and Ikaros. Helios forms homo- and heterodimers with these proteins and is thought to function predominantly in early hematopoietic development. Expression of Helios, Aiolos and Ikaros is restricted to cells of the hematopoietic system, whereas other family members, Eos and Pegassus, are more widely expressed. Helios is expressed at early stages of thymocyte development. In mature T cells, Helios has been strongly associated with Treg cells.
• Synonyms: helios; MGC34330; OTTHUMP00000163964; ZNF1A2; ZNFN1A2