Collagen II (COL2A1) Mouse Monoclonal Antibody [Clone ID: CIIC1]

CAT#: AM26375PU-N

Collagen II (COL2A1) mouse monoclonal antibody, clone CIIC1, Purified

Specifications

Product Data

• Clone Name: CIIC1
• Applications: ELISA, FN, IHC
• Recommended Dilution: Immunohistochemistry on Frozen Sections: The typical starting working dilution is 1/10.
  Immunohistochemistry on Paraffin Sections: The typical starting working dilution is 1/10.
  Functional Assays.
  Immunoassays (Coating + Detection).
• Reactivities: Bovine, Chicken, Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Specificity: The monoclonal antibody CIICI reacts with the C1 epitope (triple helical position 358-364) of collagen type II.
  It has been shown to induce CAIA in naïve mice after injection of lipopolysacharide (LPS). However, in combination with the monoclonal antibody M2139, binding to the J1 epitope of CII, the pair of monoclonal antibodies induce arthritis in different strains of mice without any other stimulants. The presence of secondary stimulus, LPS, increases the disease incidence and severity.
  The monoclonal antibody CIIC1 is cross reactive with Rat, Bovine, Chicken and Human CII.
• Formulation: PBS
  State: Purified
  State: Liquid 0.2 µm filtered Ig fraction
  Stabilizer: 0.1% BSA
• Concentration: lot specific
• Purification: Protein Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Gene Name: collagen type II alpha 1 chain
• Database Link:
  Entrez Gene 1280 Human
  UniProt ID P02458
• Background: Collagen is a structural protein in bone, cartillage and connective tissue. Collagen type II (CII) is the major collagen of the nucleus pulposa (a component of spine), cartilage and vitreous (a component of the eye). The most commonly used animal model for rheumatoid arthritis (RA) is the collagen-induced arthritis (CIA). Transfer of collagen type II specific monoclonal antibodies induces an acute form of arthritis (collagen type II antibody induced arthritis, CAIA).
• Synonyms: COL2, Collagen II