Galectin 3 (LGALS3) Mouse Monoclonal Antibody [Clone ID: B2C10]

CAT#: AM26213PU-N

Galectin 3 (LGALS3) mouse monoclonal antibody, clone B2C10, Purified

Specifications

Product Data

• Clone Name: B2C10
• Applications: ELISA, FN, IHC, WB
• Recommended Dilution: Western blotting.
  Flow Cytometry.
  Immuno Assays.
  Functional Studies: Dilutions have to be made according to the amounts of Galectin-3 to be inhibited.
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin Embedded Sections.
  Recommended Dilutions: 1/50.
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Specificity: The monoclonal antibody B2C10 reacts with Galectin-3, a 30 kDa protein.
  The monoclonal antibody B2C10 inhibits the binding of ¹²⁵I-labeled galectin-3 to IgE coated on microtiter plates, the Galecin-3’s hemagglutination activity and Galectin-3-induced superoxide production by Human neutrophils. This inhibitory activity of B2C10 is probably the result of its disruption of the self-association process.
  The epitope of the monoclonal antibody B2C10 is found within the first 45 amino acids of Galectin-3. The antibody B2C10 does not react with Galecin-3C and is cross reactive with Mouse Galectin-3.
• Formulation: PBS
  State: Purified
  State: Liquid 0.2 µm filtered Ig fraction
  Stabilizer: 0.1% BSA
• Concentration: lot specific
• Purification: Protein G Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Gene Name: lectin, galactoside binding soluble 3
• Database Link:
  Entrez Gene 3958 Human
  UniProt ID P17931
• Background:

  Galectin-3 is a member of the galectin family. The protein is composed of three domains: a small amino-terminal domain, a carboxyl-terminal carbohydrate recognition domain (CRD) and amino-terminal domain containing repeating elements. Galectin-3 is normally distributed in epithelia of many organs and various inflammatory cells, including macrophages, as well as dendritic cells and Kupffer cells. The expression of this lectin is up-regulated during inflammation, cell proliferation, cell differentiation and through trans-activation by viral proteins. The expression is also affected by neoplastic transformation: up-regulated in certain types of lymphomas and thyroid carcinoma, while down-regulated in other types of malignancies, such as colon, breast, ovarian and uterine carcinomas.
  Galectin-3 has been shown to function through both intracellular and extracellular actions. Related to its intracellular functions, galectin-3 has been identified as a component of heterogeneous nuclear ribonuclear protein (hnRNP), a factor in pre-mRNA splicing, and has been found to control cell cycle and prevent T cell apoptosis. On the other hand, this protein has also been demonstrated to function as extracellular molecule in activating various types of cells, including monocytes/macrophages, mast cells, neutrophils and lymphocytes. Galectin-3 has been shown to mediate cell-cell and cell-extracellular matrix interactions.

• Synonyms: Mac-2, Lgals3, GAL3, GALBP, CBP35, L-31