CHRM2 (168-192) Mouse Monoclonal Antibody [Clone ID: B8E5]

CAT#: AM26189PU-N

CHRM2 (168-192) mouse monoclonal antibody, clone B8E5, Purified

Specifications

Product Data

• Clone Name: B8E5
• Applications: ELISA, FN, IF, IHC, WB
• Recommended Dilution: Flow Cytometry.
  Functional Assays (Agonist-like antibody).
  Immunoassay.
  Immunofluorescence.
  Western blot (Non-Reduced Conditions, specific bands of ~55, 63 and 65 kDa).
  The typical starting working dilution is 1/50.
• Reactivities: Guinea Pig, Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human second extracellular loop residues 168-192
• Specificity: The monoclonal antibody B8E5 recognizes Human muscarinic acetylcholine receptor M2 (M2 receptor), a G protein-coupled cardiovascular receptor of ~55 kDa.
  Monoclonal antibody B8E5 inhibits the ß-adrenergic L-type Ca²⁺ currents through activation of the muscarinic acetylcholine receptor M2. It suggests that the antibody acts not via the classical pathway of decreasing cAMP, but rather by increasing cGMP.
  Monoclonal antibody B8E5 acts by functional dimerization of the receptor resulting in stabilization of the constitutive active receptor dimers and paradoxically induces a small decrease in carbachol affinity for the M2 receptor. It recognizes the pentapeptide VRTVE (aa 168-172) corresponding to the N-terminal part of the second extracellular loop of the Human M2 receptor.
• Formulation: PBS
  State: Purified
  State: Liquid 0.2 µm filtered Ig fraction
  Stabilizer: 0.1% BSA
• Concentration: lot specific
• Purification: Protein G Chromatography
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C.
  

• Stability: Shelf life: one year from despatch.

• Gene Name: cholinergic receptor muscarinic 2
• Database Link:
  Entrez Gene 1129 Human
  UniProt ID P08172
• Background: This receptor is an integral membrane protein consisting of seven membrane spanning a-helices linked together by extra- and intracellular loops that form a pharmacophore pocket. Autoantibodies directed against cardiovascular G protein-coupled receptors functionally interfering with the target have been described in several cardiovascular diseases. The M2 receptor is the predominant sybtype of muscarinic receptors present in the heart of mammalian species. The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition.
• Synonyms: CHRM2, mAChR M2, mAChR-M2