PCNA Mouse Monoclonal Antibody [Clone ID: IML-83]

CAT#: AM20660PU-N

PCNA mouse monoclonal antibody, clone IML-83, Purified

Product Images

Lane 1: MCF7 whole cell lysate Lane 2: HeLa whole cell lysate Lane 3: HT1080 whole cell lysate Lane 4: colo320 whole cell lysate

Specifications

Product Data

• Clone Name: IML-83
• Applications: IF, IHC, WB
• Recommended Dilution: Western Blot: 2 μg/ml.
  Immunohistochemistry on frozen and paraffin sections: 0.4 - 1 μg/ml.
  Immuocytochemistry.
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Protein A fusion protein
• Specificity: This antibody reacts to PCNA.
• Formulation: 1.2 % sodium acetate, with 2 mg BSA and 0.01 mg sodium azide as preservative.
  State: Purified
  State: Lyphilized purified Ig fraction
• Reconstitution Method: Restore with 1.2% sodium acetate or neutral PBS
• Concentration: 0,1 mg/ml (after reconstitution with PBS)
• Purification: Affinity chromatography
• Conjugation: Unconjugated
• Storage: Prior to reconstitution store at -20°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens proliferating cell nuclear antigen (PCNA), transcript variant 1
• Database Link:
  Entrez Gene 18538 MouseEntrez Gene 25737 RatEntrez Gene 5111 Human
  UniProt ID P12004
• Background: Proliferating cell nuclear antigen (PCNA) was originally identified by immunofluorescence as a nuclear protein whose appearance correlated with the proliferative state of the cell. PCNA /cyclin has been localized by in situ hybridization to the short arm of human chromosome 20 with a peak of grains over band 20p13. PCNA gene is present in single copy and has 6 exons. It spans 4,961 bp. Synthesis of the nuclear protein cyclin and DNA in quiescent mouse fibroblasts is coordinately induced by serum and purified growth factors. PCNA controls establishment of sister chromatid cohesion during S phase.
• Synonyms: Cyclin

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Base excision repair, Cell cycle, DNA replication, Mismatch repair, Nucleotide excision repair