GFAP Mouse Monoclonal Antibody [Clone ID: GA-8]

CAT#: AM20624PU-N

GFAP mouse monoclonal antibody, clone GA-8, Purified

Product Images

Lane 1: Rat brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: MCF7 whole cell lysate Lane 4: HeLa whole cell lysate

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  Lane 1: Rat brain tissue lysate Lane 2: Rat brain tissue lysate

Specifications

Product Data

• Clone Name: GA-8
• Applications: IHC, WB
• Recommended Dilution: Western Blot: 0.5 - 1 μg/ml.
  Immunohistochemistry on frozen sections: 0.5 - 1 μg/ml.
  Immunohistochemistry on paraffin sections: 0.4 - 1 μg/ml.
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: GFAP from pig spinal cord.
• Specificity: This antibody reacts to GFAP.
• Formulation: 1.2 % sodium acetate, with 2 mg BSA and 0.01 mg sodium azide as preservative.
  State: Purified
  State: Lyphilized purified Ig fraction
• Reconstitution Method: Restore with 1.2% sodium acetate or neutral PBS
• Concentration: 0,1 mg/ml (after reconstitution with PBS)
• Purification: Affinity chromatography
• Conjugation: Unconjugated
• Storage: Prior to reconstitution store at -20°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens glial fibrillary acidic protein (GFAP), transcript variant 2
• Database Link:
  Entrez Gene 14580 MouseEntrez Gene 24387 RatEntrez Gene 2670 Human
  UniProt ID P14136
• Background: GGlial fibrillary acidic protein (GFAP) is an intermediate-filament (IF) protein that is highly specific for cells of astroglial lineage, although its tissue-specific role is speculative. GFAP has been located in rat kidney glomeruli and peritubular fibroblasts, leydig cells of testis, skin keratinocytes, osteocytes of bones, chondrocytes of epiglottis, bronchus, and stellate-shaped cells of the pancreas and liver. Its expression is essential for normal white matter architecture and blood-brain barrier integrity, and its absence leads to late-onset CNS dysmyelination. GFAP has also been shown to play a role in mitosis by adjusting the filament network present in the cell. During mitosis, there is an increase in the amount of phosphorylated GFAP, and a movement of this modified protein to the cleavage furrow.
• Synonyms: Glial Fibrillary Acidic Protein

Reference Data

• Protein Families: ES Cell Differentiation/IPS