Ubiquitin (UBB) Mouse Monoclonal Antibody [Clone ID: 10C2-2]

CAT#: AM10231SU-N

Ubiquitin (UBB) mouse monoclonal antibody, clone 10C2-2, Supernatant

Product Images

Detection of ubiquinylated protein (Lane 1) and free (Lane 2) on cell lysate from Rat prostate.

Specifications

Product Data

• Clone Name: 10C2-2
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/10-1/100.
  Western Blot: 1/10-1/100.
  Immunohistochemistry: 1/10-1/100.
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Ubiquitin
• Specificity: The antibody reacts specifically with 8.5kDa protein and ubiquitinilated proteins.
• Formulation: State: Supernatant
  State: Lyophilized powder from Cell Culture Supernatant
  Preservative: None
• Reconstitution Method: Restore in distilled water.
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: ubiquitin B
• Database Link:
  UniProt ID P0CG47
• Background: Ubiquitin is a highly conserved protein of about 8.5 kDa molecular weight, which has an ATP dependent role in the targeting of proteins for proteolytic degradation. To perform this function, the protein to be degraded is first covalently attached to the C terminus of ubiquitin, and the ubiquitinated complex is then recognized by a complex of degradative enzymes. Interestingly, ubiquitin also becomes covalently bonded to many types of pathological inclusions, which appear to be resistant to normal degradation. Therefore, ubiquitin antibodies are very useful for studies of these inclusions. For example, the neurofibrillary tangles and paired helical filaments diagnostic of Alzheimer's disease, Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease are all heavily ubiquitinated and can be readily visualized with ubiquitin antibodies.
• Synonyms: UBB, Polyubiquitin-B