HES1 Mouse Monoclonal Antibody [Clone ID: 7C2g2]

CAT#: AM10222PU-N

HES1 mouse monoclonal antibody, clone 7C2g2, Purified

Specifications

Product Data

• Clone Name: 7C2g2
• Applications: ELISA, WB
• Recommended Dilution:

  ELISA: 1/20000-1/100000.
  Western Blot: 1/2000-1/10000.
  Immunohistochemistry.

• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic peptide derived from C terminal part of HES-1 protein
• Specificity: Reacts with Human 30kDa HES-1 protein.
  May cross react with Mouse and Rat.
• Formulation: 0.1M Tris, 0.1M Glycine and 2% Sucrose
  State: Purified
  State: Lyophilized purified powder
  Preservative: None
• Reconstitution Method: Restore in distilled water.
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage:

  Prior to reconstitution store the antibody at -20°C.
  Store reconstituted antibody at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: hes family bHLH transcription factor 1
• Database Link:
  Entrez Gene 3280 Human
  UniProt ID Q14469
• Background: HES1 is a nuclear protein belonging to th basic helix-loop-helix family of transcription factors with a basic helix-loop-helix (bHLH) domain and an Orange domain. The basic domain of HES1 contains a helix interrupting protein that binds to the N-box rather than the canonical E-box. An immediate early response gene to growth factor, HES1 is reported as having a dual function of both transcriptional activator and silencer of acid alpha-glucosidase (GAA), thus contributing to the subtle tissue-specific control of housekeeping genes such as GAA. HES1 a transcriptional repressor of genes which require a bHLH protein for their transcription and also the formation of a complex with a corepressor protein (Groucho) such as TLE2. HES1 may act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1.
• Synonyms: HES-1, HL, HRY, Hairy-like, HHL, Hairy homolog