TOLLIP Mouse Monoclonal Antibody [Clone ID: k1B17]

CAT#: AM09046PU-N

TOLLIP mouse monoclonal antibody, clone k1B17, Purified

Product Images

Cell lysates of Jurkat (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Tollip (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Western blot analysis: Cell lysates of Jurkat (30 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Tollip (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Product Data

• Clone Name: k1B17
• Applications: ELISA, IF, WB
• Recommended Dilution: ELISA.
  Western blot (1/500-1/1,000).
  Immunofluorescence.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Recombinant human Tollip (61-274 aa) purified from E. coli
• Specificity: The antibody recognizes human TOLLIP.
  Other species not tested.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein-G affinity chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: toll interacting protein
• Database Link:
  Entrez Gene 54472 Human
  UniProt ID Q9H0E2
• Background: Toll interacting protein, also known as Tollip, is an inhibitory adaptor protein within the signaling pathway of IL-1 and Toll-like receptors(TLR). The Toll-like receptors are essential transmembrane signaling receptors of the innate immune system that alert the host to the presence of a microbial invader. Tollip recruits IRAK1 to the IL-1 receptor complex and inhibits IRAK1 phosphorylation and kinase activity.
• Synonyms: Toll-interacting protein