Rhodamine Mouse Monoclonal Antibody [Clone ID: 11H10]
Specifications
Product Data | |
Clone Name | 11H10 |
Applications | ELISA, IHC, WB |
Recommended Dilution | ELISA: 1/30,000. Western blot: 1/1,000-1:10,000. Immunohistochemistry: 1/1,000-1/10,000. |
Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | Anti-RHODAMINE Monoclonal Antibody was produced after repeated immunizations of balb/c mice with rhodamine conjugated KLH. |
Specificity | This antibody reacts specifically with Rhodamine and its derivatives. Rhodamine isomer 5 and isomer 6 are reactive as TAMRA, as well as TRITC conjugated proteins. No reaction is observed against Texas Red. |
Formulation | 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 as buffer and 0.01% (w/v) Sodium Azide as preservative. State: Purified State: Liquid (sterile filtered) purified Ig fraction |
Concentration | lot specific |
Purification | Protein A Chromatography. |
Conjugation | Unconjugated |
Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C or below for longer. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use. |
Stability | Shelf life: one year from despatch. |
Background | RHODAMINE Monoclonal Antibody specifically detect dyes in the Rhodamine family. Rhodamine is a family member of the fluorone dyes. Examples are Rhodamine 6G and Rhodamine B. They are often used as a tracer dye within water to determine the rate and direction of flow and transport. Rhodamine dyes fluoresce and can thus be detected easily and inexpensively with instruments called fluorometers. Rhodamine dyes are used extensively in biotechnology applications such as fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy and ELISA. |
Synonyms | Tetramethyl Rhodamine Isothiocyanate, TRITC, TAMRA |
Reference Data |
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