Ubiquitin (UBB) Mouse Monoclonal Antibody [Clone ID: 3C12]

CAT#: AM06722PU-N

Ubiquitin (UBB) mouse monoclonal antibody, clone 3C12, Purified

Product Images

Western blot analysis using UBB antibody against human UBB (AA: 1-299) recombinant protein (Expected MW is 26 kDa).

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  Western blot analysis using UBB antibody against NIH/3T3 (1) and Hela (2) cell lysate.

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  Flow Cytometric analysis of Hela cells using UBB antibody (green) and negative control (red).

Specifications

Product Data

• Clone Name: 3C12
• Applications: ELISA, FC, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Blot: 1/500 - 1/2000.
  Flow Cytometry: 1/200 - 1/400.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of Human UBB expressed in E. Coli.
• Specificity: Recognizes UBB
• Formulation: PBS
  State: Purified
  State: Liquid purified antibody
  Stabilizer: 0.5% protein stabilizer
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 26 kDa
• Gene Name: ubiquitin B
• Database Link:
  Entrez Gene 7314 Human
  UniProt ID P0CG47
• Background: This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome.
• Synonyms: UBB, Polyubiquitin-B