AMPK alpha 1 (PRKAA1) Mouse Monoclonal Antibody [Clone ID: 2B7]

CAT#: AM06584SU-N

AMPK alpha 1 (PRKAA1) mouse monoclonal antibody, clone 2B7, Ascites

Product Images

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  Immunohistochemical analysis of paraffin-embedded ovarian cancer (left) and brain tissues (right) using PRKAA1 mouse mAb with DAB staining.

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  Immunofluorescence analysis of NTERA-2 cells using PRKAA1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

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  Flow cytometric analysis of PC-2 cells using PRKAA1 mouse mAb (green) and negative control (purple).

Specifications

Product Data

• Clone Name: 2B7
• Applications: ELISA, FC, IF, IHC, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Blot: 1/500 - 1/2000.
  Flow Cytometry: 1/200 - 1/400.
  Immunofluorescence: 1/200 - 1/1000.
  Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000.
• Reactivities: Human, Monkey, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of Human PRKAA1 expressed in E. Coli.
• Specificity: Recognizes PRKAA1
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% Sodium Azide.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 64 kDa
• Gene Name: protein kinase AMP-activated catalytic subunit alpha 1
• Database Link:
  Entrez Gene 5562 Human
  UniProt ID Q13131
• Background: The protein belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
• Synonyms: AMPK1, AMPK alpha-1 chain