ER81 (ETV1) Mouse Monoclonal Antibody [Clone ID: 1C8B6]

CAT#: AM06285SU-N

ER81 (ETV1) mouse monoclonal antibody, clone 1C8B6, Ascites

Product Images

Western blot analysis using ETV1 antibody Cat.-No AM06285SU-N against truncated Trx-ETV1 recombinant protein (Lane 1) and full-length ETV1 (aa1-477)-hIgGFc transfected CHO-K1 cell lysate (Lane 2).

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  Western blot analysis using ETV1 antibody Cat.-No AM06285SU-N against HEK293T cells transfected with the pCMV6-ENTRY control (Lane 1) and pCMV6-ENTRY ETV1 cDNA (Lane 2).

Specifications

Product Data

• Clone Name: 1C8B6
• Applications: WB
• Recommended Dilution: ELISA: 1/10000.
  Western Bloting: 1/500 - 1/2000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of ETV1 (aa1-191) expressed in E. Coli.
• Specificity: Recognizes ETV1 (Ets variant gene 1).
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% Sodium Azide.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 2115 Human
  UniProt ID P50549
• Background: Several members of the Ets gene family encode sequence-specific DNA binding proteins that recognize DNA sequences with a centrally located 5'-GGAA-3' element. All of the Ets proteins recognize the same central core sequence but each protein interacts with unique sequences that flank this core. ER81 (also designated ETV1) binds the motif 5'-CGGAA/T-3'. ER81 is highly expressed in brain, testis, lung and heart. ER81 is also moderately expressed in spleen, pancreas, colon and small intestine. During development, ER81 displays a unique expression patterns which suggests that this transcriptional factor might play an important role in organogenesis. ERK-1 activates ER81 transcriptional activity while MAPKAP kinase 2 inhibits ER81.
• Synonyms: ETS translocation variant 1, Ets-related protein 81