BCL10 Mouse Monoclonal Antibody [Clone ID: 4F8]

CAT#: AM06073PU-N

BCL10 mouse monoclonal antibody, clone 4F8, Purified

Product Images

Western blot analysis using BCL10 antibody Cat.-No AM06073PU-N against NIH/3T3 (1), Hela (2), MCF-7 (3) and Jurkat (4) cell lysate.

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  Immunohistochemical analysis of paraffin-embedded human breast carcinoma (A) and liver carcinoma (B), showing cytoplasmic localization using BCL10 antibody Cat.-No AM06073PU-N with DAB staining.

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  Confocal immunofluorescence analysis of Hela cells using BCL10 antibody Cat-No AM06073PU-N (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

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  Flow cytometric analysis of Hela cells using BCL10 antibody Cat.-No AM06073PU-N (green) and negative control (purple).

Specifications

Product Data

• Clone Name: 4F8
• Applications: ELISA, FC, IF, IHC, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Blot: 1/500 - 1/2000.
  Flow Cytometry: 1/200 - 1/400.
  Immunofluorescence: 1/200 - 1/1000.
  Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000.
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of Human BCL-10 expressed in E. Coli.
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.03% Sodium Azide
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 8915 Human
  UniProt ID O95999
• Background: Bcl-10 (B-cell CLL/lymphoma 10), also known as CLAP, Me10, CIPER, c-E10, CARMEN. Entrez Protein NP_003912. It is a protein containing a caspase recruitment domain (CARD). It plays an important role in apoptosis and activating NF-kappaB. The research suggested that it interacted with other CARD domain containing proteins including CARD9, 10, 11 and 14, which were thought to function as upstream regulators in NF-kappaB signaling. Bcl-10 is found to form a complex with MALT1 which encoded by another gene known to be translocated in MALT lymphoma. MALT1 and Bcl-10 are thought to synergize in the activation of NF-kappaB, and the deregulation of either of them may contribute to the same pathogenetic process that leads to the malignancy.
• Synonyms: BCL10, CIPER, CLAP, cCARMEN, mE10, c-E10, hCLAP