Rituximab Rat Monoclonal Antibody [Clone ID: MB2 A4]

CAT#: AM05879PU-N

Rituximab rat monoclonal antibody, clone MB2 A4, Purified

Product Images

Detection of Rituximab spiked into 10% human serum by sandwich ELISA. Human anti Rituximab was used as the coating antibody and detection was performed by adding HRP conjugated anti-Rituximab antibody AM05879PU-N. Data are shown as the mean of three indepe

Specifications

Product Data

• Clone Name: MB2 A4
• Applications: ELISA, FC
• Recommended Dilution: ELISA: 5 µg/ml as coating antibody.
  Flow Cytometry: Use 10 µl of 50 µg/ml diluted antibody to label 10⁶ cells in 100 µl.
• Reactivities: Human
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: F(ab)2 fragment of Rituximab.
  Spleen cells from immunised rats were fused with cells of the NS-1 mouse myeloma cell line
• Specificity: This antibody specifically recognises the idiotypic determinants expressed by the Rituximab humanised monoclonal antibody. It does not recognise other CD20 antibodies.
  This antibody has been used in ELISA assays to monitor the levels of Rituximab in patient serum following therapy.
  It has been used to detect Rituximab bound to the surface of the Raji B cell line, however detection of Rituximab bound 'in vivo' to B-CLL cells has not been demonstrated. It is possible that complement deposition on Rituximab opsonised cells inhibits binding of the anti-Rituximab antibody to cell bound Rituximab (3).
• Formulation: TRIS buffered saline pH 8.0
  State: Purified
  State: Liquid purified IgG fraction from Tissue Culture Supernatant
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Background: Rituximab is a therapeutic reagent directed against the human CD20 cell surface antigen.
• Synonyms: Rituxan, MabThera