SIRP alpha (SIRPA) Mouse Monoclonal Antibody [Clone ID: 15-414]

CAT#: AM05588PU-L

SIRP alpha (SIRPA) mouse monoclonal antibody, clone 15-414, Purified

Specifications

Product Data

• Clone Name: 15-414
• Applications: FC, IHC
• Recommended Dilution: Flow Cytometry: 1/25 - 1/100.
  Immunohistochemistry on frozen sections: 1/100.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Monocyte-derived dendritic cells.
• Specificity: This antibody is specific for CD172a, also known as signal-regulatory protein alpha.
• Formulation: Phosphate buffered saline pH7.4 containing 0.09% Sodium Azide (NaN3)
  State: Purified
  State: Liquid purified IgG
• Concentration: lot specific
• Purification: Affinity chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: signal regulatory protein alpha
• Database Link:
  Entrez Gene 140885 Human
  UniProt ID P78324
• Background: CD172a is a receptor-type transmembrane glycoprotein expressed on cells of myeloid origin, including granulocytes, dendritic cells (DCs), macrophages, mast cells and haematopoietic stem cells. CD172a acts as a substrate for several activated tyrosine kinases, including EGFR, PDGFR, src and insulin receptor and is involved in the negative regulation of receptor tyrosine kinase-coupled signaling pathways. Ligand binding of CD172a to integrin-associated protein CD47, results in tyrosine kinase phosphorylation of immunoreceptor tyrosine-based inhibitory motifs (ITIMs) within the cytoplasmic region of CD172a, mediating the recruitment and activation of the tyrosine phosphatases SHP-1 and SHP-2. These then act as regulators of cellular function, through dephosphorylation of specific substrates. Ligation of CD172a with CD47 has been demonstrated in several regulatory processes, including the inhibition of host cell phagocytosis by macrophages and the bi-directional activation of T cells and DCs.
• Synonyms: SHP substrate 1, SHPS-1, Sirp-alpha-2, Sirp-alpha-3, MyD-1 antigen, p84, BIT, MFR, MYD1, PTPNS1, SHPS1