Alpha SNAP (NAPA) Mouse Monoclonal Antibody [Clone ID: 15D4]

CAT#: AM05270PU-N

Alpha SNAP (NAPA) mouse monoclonal antibody, clone 15D4, Purified

Specifications

Product Data

• Clone Name: 15D4
• Applications: ELISA, IP, WB
• Recommended Dilution: Western Blot: 0.5-5 ug/ml.
  Immunoprecipitationon native and denatured samples: 2-10 ug/ml.
• Reactivities: Bovine, Human, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with recombinant human a-SNAP protein and mouse myeloma cells.
• Specificity: This antibody specifically recognizes a-SNAP as a single band of ~36 kDa on western blot of rat kidney, rat brain and MDBK cells and do not recognize b-SNAP.
• Formulation: 20 mM Sodium Phosphate, pH 7.5, 150 mM Sodium Chloride, 50% Glycerol without preservatives.
  State: Purified
  State: Liquid purified IgG fraction (> 95% pure).
• Concentration: lot specific
• Purification: Standard chromatographic techniques.
• Conjugation: Unconjugated
• Storage: Store the antibody at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: NSF attachment protein alpha
• Database Link:
  Entrez Gene 140673 RatEntrez Gene 8775 Human
  UniProt ID P54920
• Background: SNAPs (soluble NSF attachment proteins), acting in concert with SNAREs (SNAP receptors) and the N-ethylmaleimide-sensitive fusion protein (NSF), are required for the fusion of transport vesicles to their target membranes in synaptic transmission, intra-Golgi transport, endosome-to-endosome fusion and transcytotic vesicles-to-plasma membrane transport. Vesicle-to-target membrane docking (initial contact) occurs when the vesicle SNARE binds to its cognate target membrane SNARE. a-SNAP (or bSNAP in brain) then binds to this docking complex and mediates the binding of NSF and thus the formation of a 20 S fusion particle. It is thought that, once NSF is bound, ATP hydrolysis by NSF initiates the fusion process. Whereas a-SNAP is expressed in all mammalian tissues, b-SNAP is expressed only in brain.
• Synonyms: NAPA, SNAP-alpha, SNAPA