HLAE (HLA-E) Mouse Monoclonal Antibody [Clone ID: MEM-E/07]

CAT#: AM03010PU-N

HLAE (HLA-E) mouse monoclonal antibody, clone MEM-E/07, Aff - Purified

Product Images

Immunohistochemistry staining of human spleen (paraffin sections) with anti-HLA-E (clone MEM-E/07).

Specifications

Product Data

• Clone Name: MEM-E/07
• Applications: FC, IHC, IP
• Recommended Dilution: Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: spleen.
  Flow cytometry: Recommended dilution: 1-4 µg/ml
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide
• Specificity: The antibody MEM-E/07 recognized native surface-expressed HLA-E, but not denaturated heavy chain of HLA-E.
  The published results revealed that the antibody cross-reacts with some classical MHC Class I molecules (MHC Class Ia): HLA-B7 (strongly), HLA-B8 (moderately), HLA-B27, -B44 (weakly).
• Formulation: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein A chromatography (> 95% by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: major histocompatibility complex, class I, E
• Database Link:
  Entrez Gene 3133 Human
  UniProt ID P13747
• Background: HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on many types of the human cells.
• Synonyms: HLA-6.2, HLAE, MHC class I antigen E