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Home cDNA Transfection Reagents Turbofectin 8.0


High Performance Transfection with TurboFectin 8.0

The ultimate in high efficiency and low toxicity!


TurboFectin 8.0 is a new generation of transfection reagent optimized for nucleic acid delivery into eukaryotic cells. Its proprietary formulation of lipid/histone blend is supplied in 80% alcohol. TurboFectin 8.0 is the recommended transfection reagent for delivery of TrueClone/TrueORF Clone (for over-expression) and HuSH-29 constructs (shRNA for expression knock-down) in many different cell types.

  • Wide spectrum: validated on > 100 cell lines and primary cell types (Click for list)
  • Recommended for shRNA-induced silencing (click for data).
  • Preferred reagent for reverse transfection assays (click for data).
  • Simplified use: Works well in media containing antibiotic and antimycotic agents. Suitable for serum-containing media; no requirement for media change
  • Affordability: One mL can be used for 300-500 transfections of 6-well plate cultures, < $0.90 per transfection.
Catalog No. Description Price Availability  
TF81001 TurboFectin 8.0 (1 vial @ 1ml each) $420 In Stock Add to Shopping Cart
TF81005 TurboFectin 8.0 (5 vials @ 1ml each) $1650 In Stock Add to Shopping Cart


TurboFectin has been cited:

Early Changes in Cytochrome P450s and Their Associated Arachidonic Acid Metabolites Play a Crucial Role in the Initiation of Cardiac Hypertrophy Induced by Isoproterenol, Althurwi, HN;Maayah, ZH;Elshenawy, OH;El-Kadi, AO;, Drug Metab. Dispos. Aug 2015 [ SKU# TF81001]

Inhibition of dipeptidyl peptidase 8/9 impairs preadipocyte differentiation, Han, R;Wang, X;Bachovchin, W;Zukowska, Z;Osborn, JW;, Sci Rep Aug 2015 [ SKU# TF81001]

Evidence that the endosomal sorting complex required for transport-II (ESCRT-II) is required for efficient human immunodeficiency virus-1 (HIV-1) production, Meng, B;Ip, NC;Prestwood, LJ;Abbink, TE;Lever, AM;, Retrovirology Aug 2015 [ SKU# TF81001]

Slit2 Promotes Angiogenic Activity Via the Robo1-VEGFR2-ERK1/2 Pathway in Both In Vivo and In Vitro Studies, Li, S;Huang, L;Sun, Y;Bai, Y;Yang, F;Yu, W;Li, F;Zhang, Q;Wang, B;Geng, JG;Li, X;, Invest. Ophthalmol. Vis. Sci. Aug 2015 [ SKU# TF81001]

9-O-Acetylation of sialic acids is catalysed by CASD1 via a covalent acetyl-enzyme intermediate, Baumann, AM;Bakkers, MJ;Buettner, FF;Hartmann, M;Grove, M;Langereis, MA;de Groot, RJ;Mühlenhoff, M;, Nat Commun Jul 2015 [ SKU# TF81001]

Reorganization of metastamiRs in the evolution of metastatic aggressive neuroblastoma cells, Khan, FH;Pandian, V;Ramraj, S;Aravindan, S;Herman, TS;Aravindan, N;, BMC Genomics Jul 2015 [ SKU# TF81001]

Novel synthetic plasmid and doggybone™ DNA vaccines induce neutralizing antibodies and provide protection from lethal influenza challenge in mice, Scott, VL;Patel, A;Villarreal, DO;Hensley, SE;Ragwan, E;Yan, J;Sardesai, NY;Rothwell, PJ;Extance, JP;Caproni, LJ;Weiner, DB;, Hum Vaccin Immunother Jun 2015 [ SKU# TF81001]

The role of the NOD-like receptor, Nlrx1 in neuroprotection and cell death, Imbeault, E;, Thesis Jun 2015 [ SKU# TF81001]

Comparative Haploid Genetic Screens Reveal Divergent Pathways in the Biogenesis and Trafficking of Glycophosphatidylinositol-Anchored Proteins, Davis, EM;Kim, J;Menasche, BL;Sheppard, J;Liu, X;Tan, AC;Shen, J;, Cell Rep Jun 2015 [ SKU# TF81001]

CD11b(+) myeloid cells support hepatic metastasis through downregulation of Angiopoietin-like 7 in cancer cells, Lim, SY;Gordon-Weeks, A;Allen, D;Kersemans, V;Beech, J;Smart, S;Muschel, RJ;, Hepatology Apr 2015 [ SKU# TF81001]

Novel adjuvants from seaweed impede autophagy signaling in therapy-resistant residual pancreatic cancer, Aravindan, S;Ramraj, SK;Somasundaram, ST;Aravindan, N;, J. Biomed. Sci. Apr 2015 [ SKU# TF81001]

CD133 and DNA-PK regulate MDR1 via the PI3K- or Akt-NF-?B pathway in multidrug-resistant glioblastoma cells in vitro, Xi, G;Hayes, E;Lewis, R;Ichi, S;Mania-Farnell, B;Shim, K;Takao, T;Allender, E;Mayanil, CS;Tomita, T;, Oncogene Mar 2015 [ SKU# TF81001]

SIRT5 regulation of ammonia-induced autophagy and mitophagy, Polletta, L;Vernucci, E;Carnevale, I;Arcangeli, T;Rotili, D;Palmerio, S;Steegborn, C;Nowak, T;Schutkowski, M;Pellegrini, L;Sansone, L;Villanova, L;Runci, A;Pucci, B;Morgante, E;Fini, M;Mai, A;Russo, MA;Tafani, M;, Autophagy Feb 2015 [ SKU# TF81001]

MicroRNA-375 plays a dual role in prostate carcinogenesis, Costa-Pinheiro, P;, clinical epigenetics journal Jan 2015 [ SKU# TF81001]

Novel and enhanced anti-melanoma DNA vaccine targeting the tyrosinase protein inhibits myeloid-derived suppressor cells and tumor growth in a syngeneic prophylactic and therapeutic murine model, Yan, J;Tingey, C;Lyde, R;Gorham, TC;Choo, DK;Muthumani, A;Myles, D;Weiner, LP;Kraynyak, KA;Reuschel, EL;Finkel, TH;Kim, JJ;Sardesai, NY;Ugen, KE;Muthumani, K;Weiner, DB;, Cancer Gene Ther. Nov 2014 [ SKU# TF81001]

Reversal of anchorage-independent multicellular spheroid into a monolayer mimics a metastatic model, Kunjithapatham, R;Karthikeyan, S;Geschwind, JF;Kieserman, E;Lin, M;Fu, DX;Ganapathy-Kanniappan, S;, Sci Rep Oct 2014 [ SKU# TF81001]

Identification of the Major Prostaglandin Glycerol Ester Hydrolase in Human Cancer Cells, Manna, JD;Wepy, JA;Hsu, K;Chang, JW;Cravatt, BF;Marnett, LJ;, J. Biol. Chem. Oct 2014 [ SKU# TF81001]

EphrinB1 expression is dysregulated and promotes oncogenic signaling in medulloblastoma, McKinney, N;Yuan, L;Zhang, H;Liu, J;Cho, YJ;Rushing, E;Schniederjan, M;MacDonald, TJ;, J. Neurooncol. Sep 2014 [ SKU# TF81001]

Using the atomic force microscope as a nanomechanical partner to support evanescent field imaging, Amini, S;Sun, Z;Meininger, GA;Meissner, KE;, The European Physical Journal Special Topics Sep 2014 [ SKU# TF81001]

Restored immunosuppressive effect of mesenchymal stem cells on B cells after OAZ downregulation in patients with systemic lupus erythematosus, Feng, X;Che, N;Liu, Y;Chen, H;Wang, D;Li, X;Chen, W;Ma, X;Hua, B;Gao, X;Tsao, BP;Sun, L;, Arthritis & rheumatology Sep 2014 [ SKU# TF81001]

Saturation editing of genomic regions by multiplex homology-directed repair, Findlay, GM;Boyle, EA;Hause, RJ;Klein, JC;Shendure, J;, Nature Aug 2014 [ SKU# TF81001]

Lentivector integration sites in ependymal cells from a model of metachromatic leukodystrophy: non-B DNA as a new factor influencing integration, McAllister, RG;Liu, J;Woods, MW;Tom, SK;Rupar, CA;Barr, SD;, Mol Ther Nucleic Acids Aug 2014 [ SKU# TF81001]

Gene expression profiling identifies FYN as an important molecule in tamoxifen resistance and a predictor of early recurrence in patients treated with endocrine therapy, Elias, D;Vever, H;Lænkholm, AV;Gjerstorff, MF;Yde, CW;Lykkesfeldt, AE;Ditzel, HJ;, Oncogene Jun 2014 [ SKU# TF81001]

Nonviral gene transfer to human meniscal cells. Part I: transfection analyses and cell transplantation to meniscus explants, Lee, HP;Kaul, G;Cucchiarini, M;Madry, H;, Int Orthop Jun 2014 [ SKU# TF81001]

Protective immunity to H7N9 influenza viruses elicited by synthetic DNA vaccine, Yan, J;Villarreal, DO;Racine, T;Chu, JS;Walters, JN;Morrow, MP;Khan, AS;Sardesai, NY;Kim, JJ;Kobinger, GP;Weiner, DB;, Vaccine Mar 2014 [Turbofectin 8.0 SKU# TF81001]

Dopamine down-regulation of protein L-isoaspartyl methyltransferase is dependent on reactive oxygen species in SH-SY5Y cells, Ouazia, D;Levros, LC;Rassart, E;Desrosiers, RR;, Neuroscience Mar 2014 [Turbofectin 8.0 SKU# TF81001]

Posttranslational modification of mitochondrial transcription factor A in impaired mitochondria biogenesis: Implications in diabetic retinopathy and metabolic memory phenomenon, Santos, JM;Mishra, M;Kowluru, RA;, Exp. Eye Res. Mar 2014 [Turbofectin 8.0 SKU# TF81001]

Co-Administration of Molecular Adjuvants Expressing NF-Kappa B Subunit p65/RelA or Type-1 Transactivator T-bet Enhance Antigen Specific DNA Vaccine- …, Shedlock, DJ;Tingey, C;Mahadevan, L;Hutnick, N;, Vaccines Mar 2014 [Turbofectin 8.0 SKU# TF81001]

Cytochrome P450 2C Epoxygenases Mediate Photochemical Stress-induced Death of Photoreceptors, Chang, Q;Berdyshev, E;Bogaard, J;White, JJ;Chen, S;Shah, R;Mu, W;Grantner, R;Bettis, S;Grassi, MA;, J. Biol. Chem., Feb 2014. [TurboFectin 8.0 SKU# TF81001]

The Steric Gate of DNA Polymerase ι Regulates Ribonucleotide Incorporation and Deoxyribonucleotide Fidelity, Donigan, KA;McLenigan, MP;Yang, W;Goodman, MF;Woodgate, R;, J. Biol. Chem., Feb 2014. [TurboFectin 8.0 SKU# TF81001]

A Cell-Based High-Throughput Screen Addressing 3'UTR-Dependent Regulation of the MYCN Gene, Sidarovich, V;Adami, V;Quattrone, A;, Mol. Biotechnol., Feb 2014. [TurboFectin 8.0 SKU# TF81001]

The isolated carboxy-terminal domain of human mitochondrial leucyl-tRNA synthetase rescues the pathological phenotype of mitochondrial tRNA mutations in human cells, Perli, E;Giordano, C;Pisano, A;Montanari, A;Campese, AF;Reyes, A;Ghezzi, D;Nasca, A;Tuppen, HA;Orlandi, M;Di Micco, P;Poser, E;Taylor, RW;Colotti, G;Francisci, S;Morea, V;Frontali, L;Zeviani, M;d'Amati, G;, EMBO Mol Med, Jan 2014. [TurboFectin 8.0 SKU# TF81001]

UBE2Q1 expression in human colorectal tumors and cell lines, Shafiee, SM;Seghatoleslam, A;Nikseresht, M;Hosseini, SV;Alizadeh-Naeeni, M;Safaei, A;Owji, AA;, Mol. Biol. Rep. 7045-51 Nov 2013 [Turbofectin 8.0 SKU# TF81001]

A Missense Mutation in CRYBB2 Leads to Progressive Congenital Membranous Cataract by Impacting the Solubility and Function of ßB2-Crystallin, Chen, W;Chen, X;Hu, Z;Lin, H;Zhou, F;Luo, L;Zhang, X;, PLOS ONE e81290 Nov 2013 [Turbofectin 8.0 SKU# TF81001]

Targeting annexin A7 by a small molecule suppressed the activity of phosphatidylcholine-specific phospholipase C in vascular endothelial cells and inhibited atherosclerosis in apolipoprotein E????ice, Li, H;Huang, S;Wang, S;Zhao, J;Su, L;Zhao, B;Zhang, Y;Zhang, S;Miao, J;, Cell Death Dis. 2013 Sep 19;4:e806 [Turbofectin 8.0 SKU# TF81001]

Temozolomide downregulates P-glycoprotein expression in glioblastoma stem cells by interfering with the Wnt3a/glycogen synthase-3 kinase/ß-catenin pathway, Chiara Riganti, Iris Chiara Salaroglio, Valentina Caldera, Ivana Campia, Joanna Kopecka, Marta Mellai, Laura Annovazzi, Amalia Bosia, Dario Ghigo, and Davide Schiffer, Neuro Oncology, Jul 2013; 10.1093/neuonc/not104. [Turbofectin 8.0 SKU# TF81001]

Cofactor Balance by Nicotinamide Nucleotide Transhydrogenase (NNT) Coordinates Reductive Carboxylation and Glucose Catabolism in the Tricarboxylic Acid (TCA) Cycle, Paulo A. Gameiro, Laura A. Laviolette, Joanne K. Kelleher, Othon Iliopoulos, and Gregory Stephanopoulos, J. Biol. Chem., May 2013; 288: 12967 - 12977. [Turbofectin SKU# TF81001]

Cellular glutathione plays a key role in copper uptake mediated by human copper transporter 1, Edward B. Maryon, Shannon A. Molloy, and Jack H. Kaplan, Am J Physiol Cell Physiol, Apr 2013; 304: C768 - C779. [Turbofectin SKU# TF81001]

Regulation of interleukin-6 secretion by the two-pore-domain potassium channel Trek-1 in alveolar epithelial cells, Andreas Schwingshackl, Bin Teng, Manik Ghosh, Keng Gat Lim, Gabor Tigyi, Damodaran Narayanan, Jonathan H. Jaggar, and Christopher M. Waters, Am J Physiol Lung Cell Mol Physiol, Feb 2013; 304: L276 - L286. [TurboFectin SKU# TF81001]

Ubiquitin mediates the physical and functional interaction between human DNA polymerases and, Justyna McIntyre, Antonio E. Vidal, Mary P. McLenigan, Martha G. Bomar, Elena Curti, John P. McDonald, Brian S. Plosky, Eiji Ohashi, and Roger Woodgate, Nucleic Acids Res., Dec 2012; 10.1093/nar/gks1277. [Turbofectin 8.0 SKU# TF81001]

Structurally similar estradiol analogs uniquely alter the regulation of intracellular signaling pathways, James G Yarger, Robert E Babine, Michael Bittner, Erin Shanle, Wei Xu, Pamela Hershberger, and Steven H Nye, J. Mol. Endocrinol., Dec 2012; 50: 43 - 57. [Turbofectin 8.0 SKU# TF81001]

Intracellular Endothelin Type B Receptor-driven Ca2+ Signal Elicits Nitric Oxide Production in Endothelial Cells, Elena Deliu, G. Cristina Brailoiu, Karthik Mallilankaraman, Hong Wang, Muniswamy Madesh, Ashiwel S. Undieh, Walter J. Koch, and Eugen Brailoiu, J. Biol. Chem., Nov 2012; 287: 41023 - 41031. [Turbofectin 8 SKU# TF81001]

Secretomic Analysis Identifies Alpha-1 Antitrypsin (A1AT) as a Required Protein in Cancer Cell Migration, Invasion, and Pericellular Fibronectin Assembly for Facilitating Lung Colonization of Lung Adenocarcinoma Cells, Ying-Hua Chang, Shu-Hui Lee, I-Chuang Liao, Shin-Huei Huang, Hung-Chi Cheng, and Pao-Chi Liao, Mol. Cell. Proteomics, Nov 2012; 11: 1320 - 1339. [Turbofectin 8 SKU# TF81001]


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