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OriGene shRNA Recent Citations

RNA interference-mediated in vivo silencing of FasL as a strategy for the enhancement of DNA vaccine potency, Human Gene Therapy. August 2008, 19(8): 763-773 [sh-Luc(in vivo application)]

Suppression of Sproutys Has a Therapeutic Effect for a Mouse Model of Ischemia by Enhancing Angiogenesis, Koji Taniguchi, Ken-ichiro Sasaki, Kousuke Watari, Hideo Yasukawa, Tsutomu Imaizumi, Toranoshin Ayada, Fuyuki Okamoto, Takuma Ishizaki,Reiko Kato,Ri-ichiro Kohno,Hiroshi Kimura,Yasufumi Sato,Mayumi Ono,Yoshikazu Yonemitsu,and Akihiko Yoshimura, PLoS ONE. 2009; 4(5): e5467 [Spry4(in vivo application)]

MicroRNA-98 and let-7 Confer Cholangiocyte Expression of Cytokine-Inducible Src Homology 2-Containing Protein in Response to Microbial Challenge, J. Immunol., Aug 2009; 183: 1617 - 1624 [CISH]

[6]-Gingerol Suppresses Colon Cancer Growth by Targeting Leukotriene A4 Hydrolase, Cancer Res., Jul 2009; 69: 5584 - 5591 [LTA4H]

Breast Cancer Migration and Invasion Depend on Proteasome Degradation of Regulator of G-Protein Signaling 4, Cancer Res., Jul 2009; 69: 5743 - 5751. [RGS4]

DNA damage-induced phosphorylation of TRF2 is required for the fast pathway of DNA double-strand break repair, Mol. Cell. Biol., Jul 2009; 29: 3597 - 3604 [TRF2]

Dual regulation of lysophosphatidic acid (LPA1) receptor signalling by Ral and GRK, Cellular Signalling, Volume 21, Issue 7, July 2009, Pages 1207-1217 [RALa]

All Citations click here

 

Mouse Monoclonal Antibody Development

Human Proteins as Antigens
(TrueMAB™ joint-development program for human proteome)
Antigen preparation:
High Quality Antibodies Start from Antigens with Native Conformations. OriGene handles antigen preparation and provides its human protein collection for immunization. The antigen will be expressed in human HEK293T cell line and purified under native conditions with affinity chromatography to preserve native protein structures with genuine mammalian post-translational modifications. Antigens preserved protein native conformation consist of native epitopes, which result in antibodies with high specificity and reactivity. In comparison to peptide-derived antibodies, these antibodies provide exceptional performances for the immunoassays that are sensitive to protein conformation, such as immunofluorescence, immunoprecipitation, ELISA, antibody array, immunohisotchemistry and flow cytometry.
Mouse monoclonal antibody development:
  • - Balb/c mice immunization and boosting with ELISA screening
  • - Splenocyte fusion and expansion with ELISA screening
  • - Subcloning and stabilization of positive clones with ELISA screening
Antibody validation:
Extensive Validation is Critical to Generate Monoclonal Antibodies with High Reactivity for Specific Immunoassays.
  • - Western blot on OriGene overexpression cell lysate (in human cell lines)
  • - Western blot on a panel of at least 7 different cell lines
  • - IHC on up to 24 unique normal and disease-state FFPE human tissues
  • - Immunofluorescent staining on a human cell line with the over-expressed antigen
  • - Immunofluorescent staining on native cell lines
  • - ELISA using recombinant proteins generated by human cell lines

TrueMAB™ Program Details

Monoclonal antibodies for every human protein

For further questions, please contact OriGene at antibody@origene.com.

 

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