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Home Antibody All anti-c-JUN antibodies

Anti-c-JUN Antibody E254

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA300032 Rabbit Monoclonal Antibody against c-JUN (N-term) 100ul $325 In Stock
LC400825 JUN HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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WB(1)
IP(1)
IHC(1)
IF(2)
Assay(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to N-terminal residues of human c-Jun was used as immunogen. Predicted to cross-react with pig, based on sequence homology.
Clone NameE254 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, Assay, IHC, IF, IP Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:250; ICC: 1:250; IP: Use a concentration of 5 ug/ml
Predicted MW Explanation 36 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens jun proto-oncogene (JUN)
Alternative NameAP-1; AP1; c-Jun
Database LinkNP_002219
Entrez Gene 3725 Human
Entrez Gene 16476 Mouse
Entrez Gene 24516 Rat
Functionc-Jun is a component of the transcription factor AP-1 (1). The transcriptional activity of c-Jun is regulated by phosphorylation at serines 63 and 73 (1,2). Extracellular signals, including growth factors, transforming oncoproteins and UV light, stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun-dependent transcription (3,4).
Related PathwayES Cell Differentiation/IPSTranscription FactorsDruggable Genome MAPK signaling pathwayErbB signaling pathwayWnt signaling pathwayFocal adhesionToll-like receptor signaling pathwayT cell receptor signaling pathwayMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - c-Jun antibody [E254]; Anti-c-Jun [E254] antibody at 1/2000 dilution + NIH 3T3 cell lysate.Predicted band size : 36 kDa.Observed band size : 40 kDa .
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Other-Anti-c-Jun antibody [E254](TA300032); Equilibrium disassociation constant (KD)..
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Immunohistochemistry (Paraffin-embedded sections) - c-Jun antibody [E254]; Immunohistochemical analysis of cJun expression in paraffin embedded skin carcinoma tissue sample, using 1/250 TA300032.
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ICC/IF image of TA300032 stained HeLa cells. The cells were incubated with the antibody overnight at 4.. The secondary antibody (green) was , DyLight 488 goat anti-rabbit IgG (H+L) used at 1:250. for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at 1:200 for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
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ICC/IF - Anti-c-Jun antibody; TA300032 staining c-Jun in HeLa cells treated with curcumin (diferuloylmethane) , by ICC/IF.The cells were incubated at 37°C for 4h in media containing different concentrations of (curcumin (diferuloylmethane)) in DMSO. Staining of the treated cells with TA300032 (1/100 dilution) was performed overnight at 4°C. A DyLight 488 goat anti-rabbit polyclonal antibody at 1:250 was used as the secondary antibody. Nuclei were counterstained with DAPI.
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c-Jun was immunoprecipitated using 0.5mg NIH3T3 whole cell extract, 5ug of Rabbit polyclonal to c-Jun and 50ul of protein G magnetic beads (+). No antibody was added to the control (-). Secondary: Mouse monoclonalSecondary Antibody to Rabbit IgG light chain (HRP) .Band: 45kDa; c-Jun
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