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Anti-c-JUN Antibody E254
Also for c-JUN (NM_002228)
|A synthetic peptide corresponding to N-terminal residues of human c-Jun was used as immunogen. Predicted to cross-react with pig, based on sequence homology.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, ASSAY, IHC, IF, IP
||WB: 1:1000 - 1:10000; IHC-P: 1:250; ICC: 1:250; IP: Use a concentration of 5 ug/ml
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for Flow Cyt.
|Homo sapiens jun proto-oncogene (JUN)|
|AP-1; AP1; c-Jun|
|c-Jun is a component of the transcription factor AP-1 (1). The transcriptional activity of c-Jun is regulated by phosphorylation at serines 63 and 73 (1,2). Extracellular signals, including growth factors, transforming oncoproteins and UV light, stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun-dependent transcription (3,4).|
Delta-Notch Signaling Pathway
EGFR1 Signaling Pathway
MAPK signaling pathway
TGF Beta Signaling Pathway
Toll-like receptor signaling pathway
Wnt Signaling Pathway
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Western blot - c-Jun antibody [E254]; Anti-c-Jun [E254] antibody at 1/2000 dilution + NIH 3T3 cell lysate.Predicted band size : 36 kDa.Observed band size : 40 kDa .
Other-Anti-c-Jun antibody [E254](TA300032); Equilibrium disassociation constant (KD)..
Immunohistochemistry (Paraffin-embedded sections) - c-Jun antibody [E254]; Immunohistochemical analysis of cJun expression in paraffin embedded skin carcinoma tissue sample, using 1/250 TA300032.
Immunocytochemistry/ Immunofluorescence - Anti-c-Jun antibody [E254]; ICC/IF image of TA300032 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4Â°C. The secondary antibody (green) was , DyLight? 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43ÂµM.
Immunocytochemistry/ Immunofluorescence - Anti-c-Jun antibody [E254]; TA300032 staining c-Jun in HeLa cells treated with curcumin (diferuloylmethane) , by ICC/IF. Decrease in c-Jun expression correlates with increased concentration of curcumin (diferuloylmethane) as described in literature.The cells were incubated at 37Â°C for 4h in media containing different concentrations of (curcumin (diferuloylmethane)) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with TA300032 (1/100 dilution) was performed overnight at 4Â°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
Immunoprecipitation - Anti-c-Jun antibody [E254]; c-Jun was immunoprecipitated using 0.5mg NIH3T3 whole cell extract, 5Âµg of Rabbit polyclonal to c-Jun and 50Âµl of protein G magnetic beads (+). No antibody was added to the control (-). .The antibody was incubated under agitation with Protein G beads for 10min, NIH3T3 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40Âµl SDS loading buffer and incubated for 10min at 70oC; 10Âµl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with TA300032.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) .Band: 45kDa; c-Jun