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Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody


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SKU Description Datasheet Price Availability  
TA50011-100 Clone OTI4C5, Anti-DDK monoclonal antibody, 100ul $248 In Stock Add to Shopping Cart
TA50011-1 Clone OTI4C5, Anti-DDK monoclonal antibody, 1ml $590 In Stock Add to Shopping Cart
TA50011-5 Clone OTI4C5, Anti-DDK monoclonal antibody, 5ml $1700 In Stock Add to Shopping Cart
Conjugation is available for this antibody: choose conjugation type
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Product NameClone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
See all DDK (FLAG) Antibodies >>
Clone Nameclone OTI4C5 (formerly 4C5)
ImmunogenAnti-DDK monoclonal antibody is produced by immunizing mice with a synthetic peptide (DYKDDDDK) coupled to KLH
Host SpeciesMouse
FormulationPBS (pH7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide
ReactivityDDK (FLAG tag): DYKDDDDK
Validated ApplicationWB, IF, FC, IP
Suggested DilutionWB: 1:2000, IP: 1:200, IF 1:1000, FC: 1:1000
Predicted Size
Storage/StabilityStable for 1 year at -20ºC from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Aliquot to avoid repeated freezing and thawing. Aliquot will be stable at 4ºC for 3 months.
Note This antibody doesn't recognize N- terminus DDK tag. Please use TA180144 for the detection of DDK tag at any location in Western blotting.
WB Image
Immunoblot analysis of randomly picked 7 different Myc/DDK tagged overexpression lysates and Myc/DDK tagged Western molecular weight marker (MWM1001) with TA50011 at 1:2,000.
IF Image
Hela cells transiently transfected with Myc/DDK tagged GFAP ORF cDNA clone were immunostained with 4C5 anti-DDK antibody (TA50011, 1:1000), and then colored in green with Alexa 488 conjugated secondary antibody (1:2000). The nuclei (blue) were counterstained with DAPI.
IP Image
Protein purification of a human protein from over-expression lysate with anti-DDK antibody affinity column (TA50011, clone 4C5)
FC Image
HEK293T cells transfected with either Myc/DDK tagged PROM2 ORF overexpression plasmid (red) or empty vector control plasmid (blue) were immunostained with OriGene anti-DDK antibody (TA50011, 1:1000) and analyzed by flow cytometry.


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