OriGene Technologies, Inc.
Search:    
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
Home Antibody Antitag Antibodies

Clone OTI4C5, Anti-DDK monoclonal antibody

div

Specifications Citations (45) Related Products Product Documents
SKU Description Datasheet Price Availability  
TA50011-100 Clone OTI4C5, Anti-DDK monoclonal antibody, 100ul $248 In Stock Add to Shopping Cart

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details

Specification

Product NameClone OTI4C5, Anti-DDK monoclonal antibody
Clone Nameclone OTI4C5 (formerly 4C5)
IsoTypeIgG2a
Concentration1mg/ml
ImmunogenAnti-DDK monoclonal antibody is produced by immunizing mice with a synthetic peptide (DYKDDDDK) coupled to KLH
Host SpeciesMouse
FormulationPBS (pH7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide
ReactivityDDK (FLAG tag): DYKDDDDK
Validated ApplicationWB, IF, FC, IP
Suggested DilutionWB: 1:2000, IP: 1:200, IF 1:1000, FC: 1:1000
Predicted Size
Storage/StabilityStable for 1 year at -20ºC from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Aliquot to avoid repeated freezing and thawing. Aliquot will be stable at 4ºC for 3 months.
WB Image
Immunoblot analysis of randomly picked 7 different Myc/DDK tagged overexpression lysates and Myc/DDK tagged Western molecular weight marker (MWM1001) with TA50011 at 1:2,000.
WB Image
Figure from citation: Western Blot of DDK(FLAG) fused protein level by using anti-DDK antibody in WT or Selk-/- / [knockout (KO)] mice BMDMs. Dilution: 1:1000 View Citation
WB Image
Figure from citation: Western Blot of DDK(FLAG) fused protein level by using anti-DDK antibody in human LNCaP or PC-3 cells. View Citation
WB Image
Figure from citation: Western Blot of DDK(FLAG) fused protein level by using anti-DDK antibody in human Hone1 cells. View Citation
IHC Image
Figure from citation: Immunohistochemistry of DDK-SPK1 protein level by using anti-DDK antibody in cutaneous wounds of diabetic rats on day 7: (A) control group and (B) treatment group (200 magnification). Dilution: 1:2000 View Citation
IF Image
Hela cells transiently transfected with Myc/DDK tagged GFAP ORF cDNA clone were immunostained with 4C5 anti-DDK antibody (TA50011, 1:1000), and then colored in green with Alexa 488 conjugated secondary antibody (1:2000). The nuclei (blue) were counterstained with DAPI.
IF Image
Figure from citation: Immunofluorescence of DDK(FLAG) protein level by using anti-DDK antibody in tumor specimens and normal tissue derived from patients with NF2. Dilution: 1:200 View Citation
IF Image
Figure from citation: Immunofluorescence of DDK(FLAG) fused protein level by using anti-DDK antibody in mouse CAD cells. View Citation
IF Image
Figure from citation: Immunofluorescence of DDK(FLAG) fused protein level by using anti-DDK antibody in HEK cells. View Citation
IP Image
Protein purification of a human protein from over-expression lysate with anti-DDK antibody affinity column (TA50011, clone 4C5)
IP Image
Figure from citation: Immunoprecipitation of DDK(FLAG) fused protein level by using anti-DDK antibody in human CH157 NM cells. Dilution: 1:200 View Citation
IP Image
Figure from citation: Immunoprecipitation of DDK(FLAG) fused protein level by using anti-DDK antibody in HeLa cells. View Citation
FC Image
HEK293T cells transfected with either Myc/DDK tagged PROM2 ORF overexpression plasmid (red) or empty vector control plasmid (blue) were immunostained with OriGene anti-DDK antibody (TA50011, 1:1000) and analyzed by flow cytometry.

 

bar
Inc 5000 Healthcare Company
All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title