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9E10, Anti-Myc Monoclonal Antibody
|Product Name||9E10, Anti-Myc Monoclonal Antibody|
|Clone Name||clone 9E10 |
|Immunogen||AEEQKLISEEDLLRKRREQLKHKLE conjugated to KLH, corresponding to C terminal amino acids 408-432 of human c-Myc|
|Formulation||0.64 mg/ml in PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide|
|Validated Application||WB, IP, IF, FC|
|Suggested Dilution||WB: 1:500-1:1000, FC: 1:100, IF 1:100|
|Storage/Stability||Stable for 1 year at -20ºC from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Aliquot to avoid repeated freezing and thawing. Aliquot will be stable at 4ºC for 3 months.|
Immunoblot analysis of four different Myc-DDK tagged over-expression lysates and one Empty vector transfected 293T cells with TA150121 at 1:500.
Immunofluorescent staining of COS7 cells transiently transfected with Myc-DDK tagged MYD88 (Cat# RC202253).
Immunofluorescent staining of COS7 cells transiently transfected with Myc-DDK tagged RASSF1 (Cat# RC213525).
Immunofluorescent staining of COS7 cells transiently transfected with Myc-DDK tagged MEF2C (Cat# RC220584).
Figure from citation: Immunofluorescence of coexpressed wild-type(WT) HCN4 and p.Pro257Ser constructs tagged with unique C-terminal Myc tag by using anti-Myc antibody in Chinese hamster ovary cells. View Citation
Immunoprecipitation (IP) of Myc-DDK tagged HMGA2 and FCGR2A over expression lysate by anti-Myc mouse monoclonal antibody (TA150121) or with goat anti-mouse conjugated magnetic beads directly.
Figure from citation: Immunoprecipitation of Myc-fused protein level by using anti-Myc antibody in human dHL-60 cells. Dilution: 1:100 View Citation
Figure from citation: Immunoprecipitation of Myc-fused protein level by using anti-Myc antibody in HEK293 cell. View Citation
HEK293T cells transfected with either Myc-DDK tagged PA2G4 plasmid (Red) or empty vector plasmid (Blue) were immunostained with anti-Myc mouse monoclonal antibody (TA150121) at 1:100, and then analyzed by flow cytometry.