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Clone OTI2B5, Anti-His mouse monoclonal antibody

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Specifications Citations (6) Related Products Product Documents
SKU Description Datasheet Price Availability  
TA150088 Clone OTI2B5, Anti-His mouse monoclonal antibody, 400ug $248 In Stock Add to Shopping Cart

Specification

Product NameClone OTI2B5, Anti-His mouse monoclonal antibody
Clone Nameclone OTI2B5 (formerly 2B5)
IsoTypeIgG1
Concentration400ug lyophilized
ImmunogenAnti-His monoclonal antibody is produced by immunizing mice with a synthetic peptide (HHHHHH) coupled to KLH
Host SpeciesMouse
Formulation400ug lyophilized powder. The lyophilized antibody can be reconstituted with 100ul of ddH2O. The concentration after reconstitution will be 4 ug/µl.
ReactivityHis tag
Validated ApplicationWB
Suggested DilutionWB: 1:4000
Predicted Size
Storage/StabilityStable for 1 year at -20ºC from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. After reconstitution, aliquot into small quantity for long term storage at -20ºC. Avoid repeated freezing and thawing. Aliquots for short-term usage will be stable at 4ºC for 3 months.
WB Image
Anti-His Immunoblot analysis of six different HEK293T over-expression lysates containing different His tagged recombinant proteins, and two his tagged bacterial lysate produced in E.coli. at 1:4000 dilution.
WB Image
Figure from citation: Western Blot of His-fused protein level by using anti-His antibody. View Citation
Assay Image
Figure from citation: Surface Plasmon Resonance assay of His-fused protein level by using anti-His antibody in in situ protein array View Citation

 

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