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Clone OTI2B5, Anti-His mouse monoclonal antibody
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|Product Name||Clone OTI2B5, Anti-His mouse monoclonal antibody|
See all His Antibodies >>
|Clone Name||clone OTI2B5 (formerly 2B5)|
|Immunogen||Anti-His monoclonal antibody is produced by immunizing mice with a synthetic peptide (HHHHHH) coupled to KLH|
|Formulation||400ug lyophilized powder. The lyophilized antibody can be reconstituted with 100ul of ddH2O. The concentration after reconstitution will be 4 ug/µl.|
|Suggested Dilution||WB: 1:4000|
|Storage/Stability||Stable for 1 year at -20ºC from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. After reconstitution, aliquot into small quantity for long term storage at -20ºC. Avoid repeated freezing and thawing. Aliquots for short-term usage will be stable at 4ºC for 3 months.|
Anti-His Immunoblot analysis of six different HEK293T over-expression lysates containing different His tagged recombinant proteins, and two his tagged bacterial lysate produced in E.coli. at 1:4000 dilution.
Figure from citation: Western Blot of His-fused protein level by using anti-His antibody. View Citation
Figure from citation: Surface Plasmon Resonance assay of His-fused protein level by using anti-His antibody in in situ protein array View Citation