Home Antibody Antitag Antibodies
Anti-His Tag Antibody
|Product Name||2B5, Anti-His mouse monoclonal antibody|
|Immunogen||Anti-His monoclonal antibody is produced by immunizing mice with a synthetic peptide (HHHHHH) coupled to KLH|
|Formulation||400ug lyophilized powder. The lyophilized antibody can be reconstituted with 100ul of ddH2O. The concentration after reconstitution will be 4 ug/µl.|
|Suggested Dilution||WB: 1:4000|
|Storage/Stability||Stable for 1 year at -20ºC from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. After reconstitution, aliquot into small quantity for long term storage at -20ºC. Avoid repeated freezing and thawing. Aliquots for short-term usage will be stable at 4ºC for 3 months.|
Anti-His Immunoblot analysis of six different HEK293T over-expression lysates containing different His tagged recombinant proteins, and two his tagged bacterial lysate produced in E.coli. at 1:4000 dilution.